{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:59:43Z","timestamp":1790477983666,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T00:00:00Z","timestamp":1435017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health Grants","doi-asserted-by":"crossref","award":["AI090742"],"award-info":[{"award-number":["AI090742"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health Grants","doi-asserted-by":"crossref","award":["AI090902"],"award-info":[{"award-number":["AI090902"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["024\/2012"],"award-info":[{"award-number":["024\/2012"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Because of N addition and variation in the site of VDJ joining, the third complementarity\u2010determining region of the heavy chain (CDR\u2010H3) is the most diverse component of the initial immunoglobulin antigen\u2010binding site repertoire. A large component of the peritoneal cavity B\u20101 cell component is the product of fetal and perinatal B cell production. The CDR\u2010H3 repertoire is thus depleted of N addition, which increases dependency on germ\u2010line sequence. Cross\u2010species comparisons have shown that D<jats:sub>H<\/jats:sub> gene sequence demonstrates conservation of amino acid preferences by reading frame. Preference for reading frame 1, which is enriched for tyrosine and glycine, is created both by rearrangement patterns and by pre\u2010BCR and BCR selection. In previous studies, we have assessed the role of conserved D<jats:sub>H<\/jats:sub> sequence by examining peritoneal cavity B\u20101 cell numbers and antibody production in BALB\/c mice with altered D<jats:sub>H<\/jats:sub> loci. Here, we review our finding that changes in the constraints normally imposed by germ\u2010line\u2013encoded amino acids within the CDR\u2010H3 repertoire profoundly affect B\u20101 cell development, especially B\u20101a cells, and thus natural antibody immunity. Our studies suggest that both natural and somatic selection operate to create a restricted B\u20101 cell CDR\u2010H3 repertoire.<\/jats:p>","DOI":"10.1111\/nyas.12808","type":"journal-article","created":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T19:11:33Z","timestamp":1435086693000},"page":"48-56","source":"Crossref","is-referenced-by-count":6,"title":["The role of evolutionarily conserved germ\u2010line D<sub>H<\/sub> sequence in B\u20101 cell development and natural antibody production"],"prefix":"10.1111","volume":"1362","author":[{"given":"Andre M.","family":"Vale","sequence":"first","affiliation":[{"name":"Program in Immunobiology Carlos Chagas Filho Institute of Biophysics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Nobrega","sequence":"additional","affiliation":[{"name":"Department of Immunology Paulo de Goes Institute of Microbiology, Federal University of Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Harry W.","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Department of Medicine"},{"name":"Department of Microbiology"},{"name":"Department of Genetics, University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,23]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Sequences of Proteins of Immunological Interest","author":"Kabat E.A.","year":"1991"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00006-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.12.7773"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.10.007"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.10.6624"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052217"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.12.8409"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001423"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20121861"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636569"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.595"},{"key":"e_1_2_7_13_1","first-page":"43","volume-title":"The Antibodies","author":"Ivanov I.I.","year":"2002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.12.8416"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v30.i4.20"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.162.3851.369"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.153.3.694"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-004-0185-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36800"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.4049\/jimmunol.133.6.3308","article-title":"Blood clearance by anti\u2010phosphocholine antibodies as a mechanism of protection in experimental pneumococcal bacteremia","volume":"133","author":"McDaniel L.S.","year":"1984","journal-title":"J. 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