{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:20:17Z","timestamp":1758403217704},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["AM 141\/11\u20101"],"award-info":[{"award-number":["AM 141\/11\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Milk contains a variety of components that have been shown to affect the expression and localization of epithelial tight junction proteins and therefore the intestinal barrier. Thus, we hypothesized that milk would have an effect on intestinal barrier properties, owing to effects on the tight junction in an intraspecies porcine intestinal <jats:italic>in vitro<\/jats:italic> model. Jejunal samples of piglets derived from different age groups were analyzed. Transepithelial electrical resistance was recorded employing the Ussing chamber technique. Porcine milk or predigested milk in buffer solution was added to the apical side, and effects were compared to untreated controls. Unidirectional paracellular flux measurements were performed using sodium fluorescein. Tight junction protein expression and localization were analyzed by immunoblotting and immunofluorescence microscopy. Incubation with milk or predigested milk led to an increase in transepithelial electrical resistance, while paracellular permeability for sodium fluorescein did not result in significant changes. Densitometric analysis of immunoblot signals did not show significant alterations in claudin expression, but a reduction of claudin signals in apicolateral membrane compartments in both approaches became apparent via immunohistology. The functional effect might reflect a physiological protective mechanism, when offspring exclusively rely on their mother's milk and are exposed to a plethora of potentially barrier\u2010perturbing factors.<\/jats:p>","DOI":"10.1111\/nyas.13340","type":"journal-article","created":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T17:13:38Z","timestamp":1493226818000},"page":"110-118","source":"Crossref","is-referenced-by-count":4,"title":["Porcine milk induces a strengthening of barrier function in porcine jejunal epithelium <i>in vitro<\/i>"],"prefix":"10.1111","volume":"1397","author":[{"given":"Judith","family":"Radloff","sequence":"first","affiliation":[{"name":"Institute of Veterinary Physiology Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silke S.","family":"Zakrzewski","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Pieper","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander G.","family":"Markov","sequence":"additional","affiliation":[{"name":"Department of General Physiology St. Petersburg State University  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salah","family":"Amasheh","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"71","volume-title":"Biochemistry of Lactation","author":"Davies D.T.","year":"1983"},{"key":"e_1_2_10_3_1","first-page":"958","article-title":"Mechanisms of absorption enhancement by medium chain fatty acids in intestinal epithelial Caco\u20102 cell monolayers","volume":"275","author":"Lindmark T.","year":"1995","journal-title":"J. Pharmacol. Exp. Ther"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-014-1350-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.115.228312"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.115.224857"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.137588"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-009-0440-7"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00165"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1101-0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E09-01-0080"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.07.014"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.10.164"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1460-z"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520903131973"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-009-0737-8"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.070896"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.03.010"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.09.051"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0021859605005642"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006009"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.07.007"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731114000950"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-7742"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0317t.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e3181bab2d3"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0067-z"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2008.09.011"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2010.11.027"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-016-4413-x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12464"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.108.092528"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2397"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2007.08.017"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnyas.13340","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nyas.13340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T22:53:04Z","timestamp":1694299984000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1111\/nyas.13340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,26]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/nyas.13340"],"URL":"https:\/\/doi.org\/10.1111\/nyas.13340","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,26]]}}}