{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T22:41:06Z","timestamp":1774305666368,"version":"3.50.1"},"reference-count":65,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T00:00:00Z","timestamp":1500336000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS089487"],"award-info":[{"award-number":["NS089487"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is a highly neurotoxic gas. It is the second most common cause of gas\u2010induced deaths. Beyond mortality, surviving victims of acute exposure may suffer long\u2010term neurological sequelae. There is a need to develop countermeasures against H<jats:sub>2<\/jats:sub>S poisoning. However, no translational animal model of H<jats:sub>2<\/jats:sub>S\u2010induced neurological sequelae exists. Here, we describe a novel mouse model of H<jats:sub>2<\/jats:sub>S\u2010induced neurotoxicity for translational research. In paradigm I, C57\/BL6 mice were exposed to 765 ppm H<jats:sub>2<\/jats:sub>S for 40 min on day 1, followed by 15\u2010min daily exposures for periods ranging from 1 to 6 days. In paradigm II, mice were exposed once to 1000 ppm H<jats:sub>2<\/jats:sub>S for 60 minutes. Mice were assessed for behavioral, neurochemical, biochemical, and histopathological changes. H<jats:sub>2<\/jats:sub>S intoxication caused seizures, dyspnea, respiratory depression, knockdowns, and death. H<jats:sub>2<\/jats:sub>S\u2010exposed mice showed significant impairment in locomotor and coordinated motor movement activity compared with controls. Histopathology revealed neurodegenerative lesions in the collicular, thalamic, and cortical brain regions. H<jats:sub>2<\/jats:sub>S significantly increased dopamine and serotonin concentration in several brain regions and caused time\u2010dependent decreases in GABA and glutamate concentrations. Furthermore, H<jats:sub>2<\/jats:sub>S significantly suppressed cytochrome <jats:italic>c<\/jats:italic> oxidase activity and caused significant loss in body weight. Overall, male mice were more sensitive than females. This novel translational mouse model of H<jats:sub>2<\/jats:sub>S\u2010induced neurotoxicity is reliable, reproducible, and recapitulates acute H<jats:sub>2<\/jats:sub>S poisoning in humans.<\/jats:p>","DOI":"10.1111\/nyas.13419","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T16:58:40Z","timestamp":1500397120000},"page":"46-64","source":"Crossref","is-referenced-by-count":40,"title":["Characterizing a mouse model for evaluation of countermeasures against hydrogen sulfide\u2013induced neurotoxicity and neurological sequelae"],"prefix":"10.1111","volume":"1400","author":[{"given":"Poojya","family":"Anantharam","sequence":"first","affiliation":[{"name":"Department of Veterinary Diagnostic and Animal Production Medicine Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth M.","family":"Whitley","sequence":"additional","affiliation":[{"name":"Pathogenesis LLC  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Belinda","family":"Mahama","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Animal Production Medicine Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010Suk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Animal Production Medicine Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula M.","family":"Imerman","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Animal Production Medicine Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dahai","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Animal Production Medicine Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica R.","family":"Langley","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthi","family":"Kanthasamy","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson K.","family":"Rumbeiha","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Animal Production Medicine Iowa State University  Ames Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2009.08.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00147.2015"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2016.07.005"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.06580.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408448409029321"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/08958370591000618"},{"key":"e_1_2_7_8_1","first-page":"1","volume-title":"Medical and Biological Effects of Environmental Pollutants","author":"National Research Council","year":"1979"},{"key":"e_1_2_7_9_1","first-page":"655","volume-title":"Experimental and Clinical Neurotoxicology","author":"Rischitelli G.D.","year":"2000"},{"key":"e_1_2_7_10_1","unstructured":"OSHA.2016.Safety and health topics: hydrogen sulfide. Accessed June 16 2017.https:\/\/www.osha.gov\/SLTC\/hydrogensulfide\/index.html."},{"key":"e_1_2_7_11_1","unstructured":"OSHA.2016.Toxic industrial chemical guide. Vol. 2017. United States Department of Labor Washington DC."},{"key":"e_1_2_7_12_1","volume-title":"OSHA Fact Sheet","author":"OSHA"},{"key":"e_1_2_7_13_1","unstructured":"EPA.2003.Toxicological review of hydrogen sulfide.Washington DC:EPA."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.5811\/westjem.2016.9.32267"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2010.09.008"},{"key":"e_1_2_7_16_1","unstructured":"Foulkes C.H.2009.\u201cGas!\u201dThe Story of the Special Brigade. Naval and Military Press."},{"key":"e_1_2_7_17_1","article-title":"Appendix A: chemicals of interest list","author":"Department of Homeland Security","year":"2007","journal-title":"Fed. Reg"},{"issue":"223","key":"e_1_2_7_18_1","first-page":"65423","article-title":"6 CFR Part 27. Appendix to Chemical Facility anti\u2010terrorism Standards; Final Rule","volume":"72","author":"Department of Homeland Security","year":"2007","journal-title":"Fed. Reg"},{"key":"e_1_2_7_19_1","first-page":"199","article-title":"Air pollution by hydrogen sulfide in Poza Rica, Mexico; an evaluation of the incident of Nov. 24, 1950","volume":"6","author":"McCabe L.C.","year":"1952","journal-title":"AMA Arch. Ind. Hyg. Occup. Med"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4209\/aaqr.2006.09.0018"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/occmed\/48.4.255"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/occmed\/46.5.367"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-6757(95)90094-2"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1979.00500430081019"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.5144\/0256-4947.59379"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.1991.tb04967.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.32.040192.000545"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/anatox\/35.2.119","article-title":"A fatal case of acute hydrogen sulfide poisoning caused by hydrogen sulfide: hydroxocobalamin therapy for acute hydrogen sulfide poisoning","volume":"35","author":"Fujita Y.","journal-title":"J. Anal. Toxicol"},{"key":"e_1_2_7_29_1","first-page":"286","article-title":"Inhalation of hydrogen sulphide: a case of subacute manifestations and long term sequelae","volume":"48","author":"Parra O.","year":"1991","journal-title":"Br. J. Ind. Med"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.SMJ.0000072361.86796.56"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13148"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/03602530600959607"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563659709001216"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20831"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.75.3.1348"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131340"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563650.2014.990976"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00377463"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0892-0362(93)90065-V"},{"key":"e_1_2_7_40_1","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1016\/j.freeradbiomed.2010.08.028","article-title":"Neuroprotection by a mitochondria\u2010targeted drug in a Parkinson's disease model","volume":"49","author":"Ghosh A.","journal-title":"Free Radic. Biol. Med"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.40.3000"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-016-9650-4"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.10.011"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2016.04.012"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563650.2010.508044"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-62627-1.00008-1"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00380773"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/1091581810384882"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/00043764-198505000-00018"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/65.1.18"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajim.4700200109"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1080\/15563650601118010"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2005.06.016"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1977.tb03919.x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro-2016-0067"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103929"},{"key":"e_1_2_7_57_1","volume-title":"Pathology of Domestic Animals","author":"Jubb K.V.F.","year":"1992"},{"key":"e_1_2_7_58_1","first-page":"46","article-title":"Pathologic\u2013anatomic findings in experimental hydrogen sulfide poisoning (H2S). A study on rhesus monkeys","volume":"22","author":"Lund O.E.","year":"1966","journal-title":"Int. Arch. Arbeitsmed"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00201481"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1080\/14656566.2016.1258063"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1155\/NP.2003.107"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316435"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.89.139"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141223751"},{"key":"e_1_2_7_65_1","first-page":"152","article-title":"Hydrogen sulfide poisoning: an antidotal role for sodium nitrite","volume":"39","author":"Hall A.H.","year":"1997","journal-title":"Vet. Hum. Toxicol"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.11.012"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnyas.13419","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nyas.13419","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T03:28:06Z","timestamp":1696044486000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1111\/nyas.13419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":65,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1111\/nyas.13419"],"URL":"https:\/\/doi.org\/10.1111\/nyas.13419","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]}}}