{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T05:49:14Z","timestamp":1759729754233,"version":"3.41.0"},"reference-count":83,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T00:00:00Z","timestamp":1516752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dietary restriction (DR) increases median life span and protects against age\u2010related disease. Improved longevity can be achieved by restriction of dietary energy, protein, or amino acids (AAs), such as methionine (Met). Met requirements have been defined using methodologies that measure the dose response to Met when all other dietary variables are held constant and with outcomes focused on protein turnover. Here, we cover protein and sulfur AA requirements and discuss the terms \u201cdeficient,\u201d \u201coptimal,\u201d and \u201cexcess\u201d and how these need to be considered. We additionally discuss the effect of methyl\u2010donating compounds on sulfur AA metabolism and outcomes. We will discuss how the mechanistic target of rapamycin complex 1 (mTORC1) signaling network regulates protein turnover, lipogenesis and cell growth, proliferation, differentiation, and metabolism in response to hormones, AAs, and cellular energy status. Inhibition of mTORC1 signaling with rapamycin or genetic mutation increases median life span in model organisms, and mTORC1 inhibition may be responsible for some of the life span\u2013extending effects of DR. Finally, we discuss how the sulfur AAs may regulate aspects of reactive oxygen species (ROS) mitigation. Overall, we suggest that approaches evaluating AA intake need to consider whole\u2010body protein synthesis and measures related to tissue\u2010specific and whole\u2010body metabolism that have been associated with longevity.<\/jats:p>","DOI":"10.1111\/nyas.13546","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T12:32:49Z","timestamp":1516797169000},"page":"20-30","source":"Crossref","is-referenced-by-count":5,"title":["Lessons from animal nutritionists: dietary amino acid requirement studies and considerations for healthy aging studies"],"prefix":"10.1111","volume":"1418","author":[{"given":"Anna K.","family":"Shoveller","sequence":"first","affiliation":[{"name":"Department of Animal Biosciences, Ontario Agricultural College University of Guelph Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie M.","family":"McKnight","sequence":"additional","affiliation":[{"name":"Trouw Nutrition Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharine M.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, Ontario Agricultural College University of Guelph Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Cant","sequence":"additional","affiliation":[{"name":"Department of Animal Biosciences, Ontario Agricultural College University of Guelph Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,24]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/529000"},{"key":"e_1_2_14_3_1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/S0025-6196(19)30634-2","article-title":"The biology of ageing","volume":"75","author":"Holloszy J.O.","year":"2000","journal-title":"Mayo Clin. 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