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In recent years, extracellular proteins from bacteria have been found to be the major pathogenic factors for aquatic animals. The aim of this study was to systematically analyze the extracellular proteins of nine sources of <jats:italic>A. veronii<\/jats:italic> and the effects of <jats:italic>hisJ<\/jats:italic> on virulence. We screened only the common proteins from nine different sources of <jats:italic>A. veronii<\/jats:italic> by liquid chromatography\u2212tandem mass spectrometry and identified the gene <jats:italic>hisJ<\/jats:italic>. We then constructed \u0394<jats:italic>hisJ<\/jats:italic> (deleted) and C\u2010<jats:italic>hisJ<\/jats:italic> (complemented) variants of <jats:italic>A. veronii<\/jats:italic> TH0426 to assess the biological function of <jats:italic>hisJ<\/jats:italic>. While the \u0394<jats:italic>hisJ<\/jats:italic> strain did not show altered growth (<jats:italic>P<\/jats:italic> &gt; 0.05), we observed that it had reduced colony formation and biofilm formation and reduced adhesion to and invasion of epithelioma papulosum cyprini cells by 2.0\u2010, 1.9\u2010, and 10.8\u2010fold, respectively. Additionally, infection experiments on zebrafish and mouse infection experiments showed that the virulence of the \u0394<jats:italic>hisJ<\/jats:italic> strain was decreased by 865\u2010fold (<jats:italic>P<\/jats:italic> &lt; 0.001) compared with the wild\u2010type strain; virulence of the complemented C\u2010<jats:italic>hisJ<\/jats:italic> strain was reduced only 2.8\u2010fold. Furthermore, in the context of <jats:italic>hisJ<\/jats:italic> deletion, flagella of <jats:italic>A. veronii<\/jats:italic> TH0426 were easily detached and the expression of virulence genes was downregulated. A persistence test (of bacterial colonies in crucian carp) showed that the number of bacteria in the immune organs of the \u0394<jats:italic>hisJ<\/jats:italic>\u2010infected group was lower than that in the wild\u2010type\u2013infected group. Overall, these results show that <jats:italic>hisJ<\/jats:italic> affects flagellar shedding, virulence, biofilm formation, adhesion, and invasion of <jats:italic>A. veronii<\/jats:italic> TH0426, and that <jats:italic>hisJ<\/jats:italic> is closely associated with virulence and plays a crucial role in its pathogenicity of <jats:italic>A. veronii<\/jats:italic> TH0426.<\/jats:p>","DOI":"10.1111\/nyas.14265","type":"journal-article","created":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T12:16:55Z","timestamp":1572437815000},"page":"146-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Functional analysis of <i>hisJ<\/i> in <i>Aeromonas veronii<\/i> reveals a key role in virulence"],"prefix":"10.1111","volume":"1465","author":[{"given":"Hai\u2010peng","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010huan","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling\u2010cong","family":"Kong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u2010qi","family":"Ju","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010ming","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inam","family":"Muhammad","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai\u2010dong","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010feng","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010xia","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05214-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90121-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.8.3611-3617.1988"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.07.006"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.04.023"},{"key":"e_1_2_9_7_1","first-page":"35","article-title":"Progress of study on membrane protein of Gram negative bacteria","volume":"21","author":"Yanrong Y.U.","year":"2000","journal-title":"Progr. 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