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Rats were exposed to aerosolized CEES and euthanized 12 h later for collection of tissue and plasma. Profiling of miRNAs in plasma, using a TaqMan\u2010based RT\u2010PCR array, revealed 14 differentially expressed miRNAs. Target gene prediction and pathway analysis revealed miRNA\u2010mediated regulation of organismal injury, inflammation, and respiratory diseases. miR\u2010140\u20105p, a marker of ALI, was downregulated in the plasma, lung, liver, and kidney of CEES\u2010exposed rats, with a concomitant increase in the expression of the inflammation markers IL\u20106 and IL\u20101\u03b1 and the coagulation marker tissue factor (F3). Exposure of rat airway epithelial cells (RL\u201065) to CEES (0.5 mM) caused cell death and a decrease in miR\u2010140\u20105p both in cells and media supernatant. This was accompanied by an increase in cellular mRNA levels of IL\u20106, IL\u20101\u03b1, and F3, as well as FGF9 and EGR2, putative targets of miR\u2010140. Knockdown of miR\u2010140 by specific oligos in RL\u201065 cells mimicked the <jats:italic>in vivo<\/jats:italic> CEES\u2010mediated effects, leading to significantly increased mRNA levels of IL\u20106, IL\u20101\u03b1, F3, FGF9, and EGR2. Our study identifies miR\u2010140\u20105p as a mediator of CEES\u2010induced ALI, which could potentially be targeted for therapy.<\/jats:p>","DOI":"10.1111\/nyas.14416","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T01:10:20Z","timestamp":1593479420000},"page":"148-158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["MicroRNA\u2010mediated inflammation and coagulation effects in rats exposed to an inhaled analog of sulfur mustard"],"prefix":"10.1111","volume":"1479","author":[{"given":"Tapasi","family":"Rana","sequence":"first","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aamir","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iram","family":"Zafar","sequence":"additional","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nithya","family":"Mariappan","sequence":"additional","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darshan Shimoga","family":"Chandrashekar","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq","family":"Hamid","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maroof","family":"Husain","sequence":"additional","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sooryanarayana","family":"Varambally","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1049-5054","authenticated-orcid":false,"given":"Shama","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0686-0987","authenticated-orcid":false,"given":"Aftab","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Division of Molecular and Translational Biomedicine Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194530"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.341.6150.1051"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)79079-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2005.01945.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2015.08.1105"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1504690"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.4570.290"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS13192J"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2009.04.060"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.jom.0000094993.20914.d1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13095"},{"key":"e_1_2_8_13_1","unstructured":"Kimmel E.C.&K.R.Still. 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