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These result in stress\u2010induced ventricular arrhythmic episodes, with clinical symptoms and prognosis reported more severe in male than female patients. Murine homozygotic <jats:italic>RyR2\u2010P2328S<\/jats:italic> (<jats:italic>RyR2<jats:sup>S\/S<\/jats:sup><\/jats:italic>) hearts replicate the proarrhythmic CPVT phenotype of abnormal sarcoplasmic reticular Ca<jats:sup>2+<\/jats:sup> leak and disrupted Ca<jats:sup>2+<\/jats:sup> homeostasis. In addition, <jats:italic>RyR2<jats:sup>S\/S<\/jats:sup><\/jats:italic> hearts show decreased myocardial action potential conduction velocities (CV), all features implicated in arrhythmic trigger and substrate. The present studies explored for independent and interacting effects of <jats:italic>RyR2<jats:sup>S\/S<\/jats:sup><\/jats:italic> genotype and sex on expression levels of molecular determinants of Ca<jats:sup>2+<\/jats:sup> homeostasis (CASQ2, FKBP12, SERCA2a, NCX1, and Ca<jats:sub>V<\/jats:sub>1.2) and CV (Na<jats:sub>V<\/jats:sub>1.5, Connexin (Cx)\u201043, phosphorylated\u2010Cx43, and TGF\u2010\u03b21) in mice. Expression levels of Ca<jats:sup>2+<\/jats:sup> homeostasis proteins were not altered, hence implicating abnormal RyR2 function alone in disrupted cytosolic Ca<jats:sup>2+<\/jats:sup> homeostasis. Furthermore, altered Na<jats:sub>V<\/jats:sub>1.5, phosphorylated Cx43, and TGF\u2010\u03b21 expression were not implicated in the development of slowed CV. By contrast, decreased Cx43 expression correlated with slowed CV, in female, but not male, <jats:italic>RyR2<jats:sup>S\/S<\/jats:sup><\/jats:italic> mice. The CV changes may reflect acute actions of the increased cytosolic Ca<jats:sup>2+<\/jats:sup> on Na<jats:sub>V<\/jats:sub>1.5 and Cx43 function.<\/jats:p>","DOI":"10.1111\/nyas.14426","type":"journal-article","created":{"date-parts":[[2020,7,26]],"date-time":"2020-07-26T18:56:28Z","timestamp":1595789788000},"page":"63-74","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Protein expression profiles in murine ventricles modeling catecholaminergic polymorphic ventricular tachycardia: effects of genotype and sex"],"prefix":"10.1111","volume":"1478","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9166-9249","authenticated-orcid":false,"given":"Khalil","family":"Saadeh","sequence":"first","affiliation":[{"name":"Faculty of Health and Medical Sciences University of Surrey  Guildford United Kingdom"},{"name":"School of Clinical Medicine, Addenbrooke's Hospital University of Cambridge  Cambridge United 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