{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:15:07Z","timestamp":1790792107814,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871219:82171649"],"award-info":[{"award-number":["81871219:82171649"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2021YFC2701003:2021YFC2701104"],"award-info":[{"award-number":["2021YFC2701003:2021YFC2701104"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nyaspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonsyndromic cleft lip with palate (nsCLP) is a common congenital malformation; however, early prenatal diagnosis is challenging and pathogenesis remains unclear. The purpose of this study was to determine the diagnostic potential of miRNAs in plasma\u2010derived exosomes and whole plasma of pregnant women to identify nsCLP and an underlying mechanism. Combined RNA sequencing analysis was performed on samples from plasma exosomes and whole plasma of pregnant women carrying normal fetuses or fetuses with nsCLP in an ongoing birth cohort, in addition to lip samples from nsCLP fetuses and healthy controls. Eight let\u20107 cluster miRNAs (hsa\u2010let\u20107a\u20103p, hsa\u2010let\u20107a\u20105p, hsa\u2010let\u20107c\u20105p, hsa\u2010let\u20107d\u20103p, hsa\u2010let\u20107d\u20105p, hsa\u2010let\u20107e\u20105p, hsa\u2010let\u20107f\u20105p, and hsa\u2010miR\u201098\u20105p) in plasma exosomes from pregnant women provided higher sensitivity\/specificity for diagnosing fetal nsCLP than those in plasma. Area under the receiver operating characteristic curve value of the eight miRNAs from plasma exosomes was 0.992. Among them, hsa\u2010let\u20107a\u20103p showed better diagnostic capability and was downregulated in nsCLP fetal lip tissues. Upstream and downstream target genes of hsa\u2010let\u20107a\u20103p were screened and confirmed. Our work highlights the potential clinical application value of let\u20107 clusters in predicting nsCLP and associates as a new regulatory axis (EN2\u2013LIN28A\u2013hsa\u2010let\u20107a\u20103p\u2013HHIP\u2013GLI2) with human nsCLP pathogenesis.<\/jats:p>","DOI":"10.1111\/nyas.14868","type":"journal-article","created":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T05:45:38Z","timestamp":1658295938000},"page":"234-246","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Identification by RNA\u2010Seq of let\u20107 clusters as prenatal biomarkers for nonsyndromic cleft lip with palate"],"prefix":"10.1111","volume":"1516","author":[{"given":"Shanshan","family":"Jia","sequence":"first","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"},{"name":"Department of Pulmonary and Critical Care Medicine, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"},{"name":"Department of Ultrasound, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9976-6748","authenticated-orcid":false,"given":"Hui","family":"Gu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenting","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0967-9462","authenticated-orcid":false,"given":"Zhengwei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital China Medical University  Shenyang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2933"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh052"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60695-4"},{"key":"e_1_2_9_5_1","volume-title":"Cleft lip","author":"Walker N. 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