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An ARM rat model was induced using ethylenethiourea. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization and immunofluorescence were used to detect the spatiotemporal expression of miR\u2010141\u20103p and Ubtd2, respectively. A dual\u2010luciferase reporter assay confirmed their targeting relationship, and cell proliferation and apoptosis were investigated after transfection in the intestinal epithelium (IEC\u20106). Additionally, western blotting and co\u2010immunoprecipitation were used to examine the protein levels and the endogenous binding relationship.\u00a0miR\u2010141\u20103p was downregulated in the ARM group, whereas Ubtd2 increased and colocalized with TUNEL\u2010positive cells.\u00a0After miR\u2010141\u20103p inhibition, protein expression of USP5 and \u03b2\u2010catenin was affected via Ubtd2, and USP5 could bind to both Ubtd2 and \u03b2\u2010catenin. Flow cytometry analysis\u00a0and\u00a0caspase 3\/7 staining demonstrated that downregulated\u00a0miR\u2010141\u20103p promoted cell apoptosis through Ubtd2. In summary, targeting Ubtd2 decreased in miR\u2010141\u20103p and promoted apoptosis of intestinal epithelium and regulated \u03b2\u2010catenin expression. This may cause aberrant apoptosis during hindgut development and mediate the imbalance of \u03b2\u2010catenin signaling in the cloaca, further affecting the occurrence of ARMs.<\/jats:p>","DOI":"10.1111\/nyas.14924","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T02:55:42Z","timestamp":1667530542000},"page":"315-327","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["miR\u2010141\u20103p affects \u03b2\u2010catenin signaling\u00a0and apoptosis by targeting Ubtd2 in rats with anorectal malformations"],"prefix":"10.1111","volume":"1518","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0944-3222","authenticated-orcid":false,"given":"Chen Yi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Shengjing Hospital of China Medical University  Shenyang PR China"},{"name":"Key Laboratory of Health Ministry for Congenital Malformation Shengjing 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