{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:30:38Z","timestamp":1792269038165,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:00:00Z","timestamp":1675123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018956","name":"NIHR Cambridge Biomedical Research Centre","doi-asserted-by":"publisher","award":["IS\u2010BRC\u20101215\u201020014"],"award-info":[{"award-number":["IS\u2010BRC\u20101215\u201020014"]}],"id":[{"id":"10.13039\/501100018956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nyaspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Thiamine (vitamin B1) is an essential micronutrient required as a cofactor in many metabolic processes. Clinical symptoms of thiamine deficiency are poorly defined, hence biomarkers of thiamine status are important. The erythrocyte transketolase activity coefficient (ETKac) is a sensitive measure of thiamine status, but its interpretation may be confounded where the availability of the transketolase enzyme is limited. Basal ETK activity per gram of hemoglobin provides a complementary biomarker of thiamine status; however, its measurement and calculation are poorly described. Here, we describe in detail the assessment of basal ETK activity, including the calculation of path length in microplates and the molar absorption coefficient of NADH specific to the assay, and the measurement of hemoglobin in sample hemolysates. To illustrate the application of the methods, we present ETKac and basal ETK activity from women in The Gambia and UK. In conclusion, we present a clear protocol for the measurement of basal ETK activity that will permit the harmonization of methods to improve replication between laboratories.<\/jats:p>","DOI":"10.1111\/nyas.14962","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T11:09:24Z","timestamp":1675163364000},"page":"104-111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Protocol and application of basal erythrocyte transketolase activity to improve assessment of thiamine status"],"prefix":"10.1111","volume":"1521","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7380-9797","authenticated-orcid":false,"given":"Kerry S.","family":"Jones","sequence":"first","affiliation":[{"name":"Nutritional Biomarker Laboratory, MRC Epidemiology Unit University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damon A.","family":"Parkington","sequence":"additional","affiliation":[{"name":"Nutritional Biomarker Laboratory, MRC Epidemiology Unit University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan W.","family":"Bourassa","sequence":"additional","affiliation":[{"name":"Micronutrient Forum  Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Cerami","sequence":"additional","affiliation":[{"name":"Faculty of Epidemiology and Population Health, Nutrition and Planetary Health Theme MRC Unit The Gambia at the London School of Hygiene &amp; Tropical Medicine  Banjul Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Koulman","sequence":"additional","affiliation":[{"name":"Nutritional Biomarker Laboratory, MRC Epidemiology Unit University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,31]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14536"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13919"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/mcn.12397"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14610"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/20.6.528"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.11.1867"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1962.tb30574.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/32.11.2237"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/33.4.848"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/30.10.1591"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19760030"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/20.9.946"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00320-9"},{"key":"e_1_2_10_15_1","unstructured":"Whitfield K. 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