{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:59:06Z","timestamp":1787317146483,"version":"build-2736575974"},"reference-count":82,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["nyaspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann NY Acad Sci"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Myogenesis is essential for skeletal muscle formation, growth, and regeneration and can be altered in Duchenne muscular dystrophy (DMD), an X\u2010linked disorder due to the absence of the cytoskeletal protein dystrophin. Ion channels play a pivotal role in muscle differentiation and interact with the dystrophin complex. To investigate ion channel involvement in myogenesis in dystrophic settings, we performed electrophysiological characterization of two immortalized mouse cell lines, wild\u2010type (WT) H2K\u20102B4 and the dystrophic (DYS) H2K\u2010SF1, and measured gene expression of differentiation markers and ion channels. Inward and outward currents\/density increased as differentiation progressed in both WT and DYS cells. However, day\u201011 DYS cells showed higher (27%) inward current density with an increased expression ratio of\n                    <jats:italic>Scn5a<\/jats:italic>\n                    \/\n                    <jats:italic>Scn4a<\/jats:italic>\n                    and decreased (48%) barium\u2010sensitive outward current compared to WT. Furthermore, day\u201011 DYS cells showed more positive resting membrane potential (+10\u00a0mV) and lower membrane capacitance (50%) compared to WT. DYS cells also had reduced\n                    <jats:italic>Myog<\/jats:italic>\n                    and\n                    <jats:italic>Myf5<\/jats:italic>\n                    expression at days 6 and 11. Overall, ion channel profile and myogenesis appeared altered in DYS cells. These results are a first step in validating ion channels as potential drug targets to ameliorate muscle degeneration in DMD settings and as differentiation biomarkers in innovative platforms.\n                  <\/jats:p>","DOI":"10.1111\/nyas.15124","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T12:49:26Z","timestamp":1711111766000},"page":"130-144","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ion channels as biomarkers of altered myogenesis in myofiber precursors of Duchenne muscular dystrophy"],"prefix":"10.1111","volume":"1534","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6836-3965","authenticated-orcid":false,"given":"Alessandro Giovanni","family":"Cerchiara","sequence":"first","affiliation":[{"name":"Department of Pharmacy \u2013 Drug Sciences University of Bari Aldo Moro  Bari 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