{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:34:46Z","timestamp":1783960486038,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T00:00:00Z","timestamp":1719014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["nyaspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate the protective effect of NAcM\u2010OPT, a small molecule inhibitor of defective in cullin neddylation 1 (DCN1), on H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced oxidative damage in keratinocytes. Immortalized human keratinocytes (HaCaT cells) were treated with NAcM\u2010OPT and exposed to oxidative stress. CCK\u20108 assays were used to measure cell viability. The mGFP\u2010RFP\u2010LC3 dual fluorescent autophagy indicator system was utilized to evaluate changes in autophagic flux. Western blotting was used to measure the expression of the autophagy\u2010related proteins LC3 and Beclin 1. Keratinocytes were treated with the autophagy activator rapamycin, and HaCaT cell supernatant was added to PIG1 cells (immortalized human melanocytes), followed by evaluation of tyrosinase (\n                    <jats:italic>TYR<\/jats:italic>\n                    ) expression via qRT\u2010PCR. NAcM\u2010OPT increased cell viability and cell proliferation. Furthermore, this molecule promoted autophagic flux through increased expression of autophagy\u2010related proteins under H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced oxidative stress. Additionally, rapamycin increased the mRNA levels of\n                    <jats:italic>TYR<\/jats:italic>\n                    in PIG1 cells. Moreover, NAcM\u2010OPT alleviated mitochondrial damage, restored mitochondrial function, and upregulated the expression of\n                    <jats:italic>NFE2L2<\/jats:italic>\n                    ,\n                    <jats:italic>HO1<\/jats:italic>\n                    ,\n                    <jats:italic>NQO1<\/jats:italic>\n                    , and\n                    <jats:italic>GCLM<\/jats:italic>\n                    . Importantly, NAcM\u2010OPT also increased epidermal thickness, follicle length, and melanin synthesis under oxidative stress in vivo. These findings suggest that NAcM\u2010OPT may be a promising small molecule antioxidant drug for the treatment of vitiligo.\n                  <\/jats:p>","DOI":"10.1111\/nyas.15173","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T13:34:31Z","timestamp":1719063271000},"page":"155-167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["NAcM\u2010OPT protects keratinocytes from H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    \u2010induced cell damage by promoting autophagy"],"prefix":"10.1111","volume":"1537","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7639-5326","authenticated-orcid":false,"given":"Renxue","family":"Xiong","sequence":"first","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"},{"name":"Department of Dermatology Hangzhou Third People's Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmei","family":"Shen","sequence":"additional","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Li","sequence":"additional","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Jin","sequence":"additional","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingru","family":"Dong","sequence":"additional","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuzu","family":"Song","sequence":"additional","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"},{"name":"Department of Dermatology Hangzhou Third People's Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiping","family":"Guan","sequence":"additional","affiliation":[{"name":"Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"},{"name":"Department of Dermatology Hangzhou Third People's Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60763-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2020.09.088"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2015.09.003"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13858"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.65"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13868"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.12.012"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-0594"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2022.11227"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3727\/096504017X14920318811712"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01243-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b01282"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2386"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-014-0104-6"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11705"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22235-8"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101133"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v26.i39.5997"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-023-03717-5"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02147-12"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000336777"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.09.023"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.8976.1"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13542"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.037"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14732"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.03.015"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2020.144854"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14585"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.04.004"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a008706"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2009.12.008"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.10.026"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450117666160819155028"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-019-0512-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42394"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2011.11.030"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.11.061"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.126"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/01913123.2022.2044946"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/01913123.2016.1218987"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20141450"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.203729"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12893"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nyas.15173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T10:02:33Z","timestamp":1721124153000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1111\/nyas.15173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,22]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/nyas.15173"],"URL":"https:\/\/doi.org\/10.1111\/nyas.15173","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/NYAS.15173\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/NYAS.15173\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/NYAS.15173\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/NYAS.15173\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/NYAS.15173\/v2\/decision1","asserted-by":"object"}]},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,22]]},"assertion":[{"value":"2024-06-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}