{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:28:53Z","timestamp":1790166533504,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,10,29]],"date-time":"2015-10-29T00:00:00Z","timestamp":1446076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Structured Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the effects of local delivery of recombinant fusion protein osteoprotegerin (<jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\u2010Fc) and bisphosphonate zoledronate on bone and periodontal ligament in a rat tooth movement model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Maxillary first molars of 36 male Sprague\u2013Dawley rats were displaced mesially using a calibrated spring connected to an anterior mini\u2010screw. Two different drugs were used: a single dose of Zoledronate (16\u00a0\u03bcg) and a twice\u2010weekly dose of <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\u2010Fc (5.0\u00a0mg\/kg) were injected. Tooth movement was measured on scanned plaster casts. Structural and immunohistochemical analysis of the orthodontic\u2010induced changes in bone included receptor activator of nuclear factor \u0138 (<jats:styled-content style=\"fixed-case\">RANK<\/jats:styled-content>), Runx, type 1 collagen, matrix metalloproteinases (MMPs) 2 and 9, tissue inhibitors of metalloproteinases (TIMPs) 1 and 2, and vimentin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both groups showed a reduction in mesial molar displacement. Animals receiving <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\u2010Fc demonstrated only 52%, 31%, and 21% of the total mesial molar displacement compared to control rats at 7, 14, and 21\u00a0days, respectively (*<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001). For rats receiving zoledronate tooth displacement decreased significantly with 52%, 46% and 30%, respectively (*<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001). At 14 and 21\u00a0days, <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\u2010Fc group showed significantly less molar displacement than the zoledronate group (*<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). <jats:styled-content style=\"fixed-case\">RANK<\/jats:styled-content>, Runx, vimentin, MMP\u20109 and tissues\u2010inhibitor metalloproteinase 1 immunoreactivity were reduced in zoledronate treated animals and even more in <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content> treated animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Local delivery of <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\u2010Fc or zoledronate inhibits bone resorption and therefore tooth movement. <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>\u2010Fc was more effective than zoledronate in blocking the action of osteoclasts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ocr.12115","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T04:51:07Z","timestamp":1446180667000},"page":"54-64","source":"Crossref","is-referenced-by-count":15,"title":["Osteoprotegerin and zoledronate bone effects during orthodontic tooth movement"],"prefix":"10.1111","volume":"19","author":[{"given":"F. 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