{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T10:13:55Z","timestamp":1769422435784,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:00:00Z","timestamp":1553040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["201608080067 to J.S."],"award-info":[{"award-number":["201608080067 to J.S."]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Objectives were (a) to establish an in vitro coculture model of human <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>\u2010derived fibroblasts (<jats:styled-content style=\"fixed-case\">HPDF<\/jats:styled-content>s) and human alveolar bone\u2010derived osteoblasts (<jats:styled-content style=\"fixed-case\">HABO<\/jats:styled-content>s), and (b) to measure <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic> and <jats:italic>P2<jats:styled-content style=\"fixed-case\">RX<\/jats:styled-content>7<\/jats:italic> gene expression at multiple time points after compressive force (<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>) stimulation for different durations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting\/Sample population<\/jats:title><jats:p>Human <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>\u2010derived fibroblasts and <jats:styled-content style=\"fixed-case\">HABO<\/jats:styled-content>s from two individuals isolated from extracted teeth of healthy donors due to orthodontic reasons at LMU's Dental School were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Mono\u2010 and cocultured cells were subjected to <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> by centrifugation (47.4\u00a0g\/cm<jats:sup>2<\/jats:sup>) for 1, 2 and 4\u00a0hours at 30\u00b0C. Total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was isolated before and 2, 4, 8, 16\u00a0minutes after <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> application. Expression of <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic> and <jats:italic>P2<jats:styled-content style=\"fixed-case\">RX<\/jats:styled-content>7<\/jats:italic> was determined using quantitative real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In monocultured cells, an upregulation of <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic> and <jats:italic>P2<jats:styled-content style=\"fixed-case\">RX<\/jats:styled-content>7<\/jats:italic> expression was found after all <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> durations: <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic> twofold in <jats:styled-content style=\"fixed-case\">HPDF<\/jats:styled-content>s after 4\u00a0hours (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002) and 1.7\u2010fold in <jats:styled-content style=\"fixed-case\">HABO<\/jats:styled-content>s after 1\u00a0hour (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.009) or 2\u00a0hours (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002) of <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>. <jats:italic>P2<jats:styled-content style=\"fixed-case\">RX<\/jats:styled-content>7<\/jats:italic> was significantly upregulated following 1\u00a0hour (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.004) and 2\u00a0hours (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002) of <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> application in <jats:styled-content style=\"fixed-case\">HPDFS<\/jats:styled-content>. In <jats:styled-content style=\"fixed-case\">HABO<\/jats:styled-content>s, a transient regulation was observed. In cocultured cells, a significant alleviation in gene expression was found for both loci after <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> application. In both cell types, <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic> expression was significantly reduced in cocultured cells compared to cells in monoculture. <jats:italic>P2<jats:styled-content style=\"fixed-case\">RX<\/jats:styled-content>7<\/jats:italic> expression showed significant attenuation in <jats:styled-content style=\"fixed-case\">HPDF<\/jats:styled-content>s only.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cocultivation attenuated the <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> induced increase of <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2<\/jats:italic> and <jats:italic>P2<jats:styled-content style=\"fixed-case\">RX<\/jats:styled-content>7<\/jats:italic> gene expression in comparison to monoculture in both cell types. This suggests that intercellular communication may exist between <jats:styled-content style=\"fixed-case\">HPDF<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">HABO<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ocr.12307","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T07:50:47Z","timestamp":1551685847000},"page":"168-176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Differences in <i><scp>RUNX<\/scp>2<\/i> and <i>P2<scp>RX<\/scp>7<\/i> gene expression between mono\u2010 and coculture of human periodontal ligament cells and human osteoblasts under compressive force application"],"prefix":"10.1111","volume":"22","author":[{"given":"Jianwei","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics University Hospital LMU Munich Munich Germany"},{"name":"Key Laboratory of Oral Medicine Guangzhou Institute of Oral Disease Stomatology Hospital of Guangzhou Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Folwaczny","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Periodontology University Hospital LMU Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8489-167X","authenticated-orcid":false,"given":"Andrea","family":"Wichelhaus","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics University Hospital LMU Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4693-6969","authenticated-orcid":false,"given":"Uwe","family":"Baumert","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics University Hospital LMU Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2016.06.021"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22086"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.05.024"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1601-6335.2003.00270.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109881200"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2009.02.013"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-14-0226"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24863"},{"issue":"10","key":"e_1_2_8_10_1","first-page":"643","article-title":"Effects of continuous and interrupted forces on gene transcription in periodontal ligament cells in vitro","volume":"49","author":"Hacopian N","year":"2011","journal-title":"Acta Med Iran"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345930720091601"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(97)00108-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-018-2697-2"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v024a00"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1142\/7018"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4725"},{"issue":"4","key":"e_1_2_8_17_1","first-page":"402","article-title":"Analysis of relative gene expression data using real\u2010time quantitative PCR and the 2\u2212\u0394\u0394C\n                  T method","volume":"25","author":"Livak KJ","year":"2001","journal-title":"Methods (Amsterdam, Neth)"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3758\/BRM.41.4.1149"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.2010.00886.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-013-1032-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.10.006"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2016.05.001"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-013-0810-x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2011.02.016"},{"issue":"11","key":"e_1_2_8_25_1","first-page":"14596","article-title":"Co\u2010culture with periodontal ligament stem cells enhanced osteoblastic differentiation of MC3T3\u2010E1 cells and osteoclastic differentiation of RAW264.7 cells","volume":"8","author":"Chen S","year":"2015","journal-title":"Int J Clin Exp Pathol"}],"container-title":["Orthodontics &amp; 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