{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T14:22:17Z","timestamp":1777904537271,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T00:00:00Z","timestamp":1557446400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Enamel organ epithelium (EOE) gives rise to the major epithelial\u2010derived cell types of tooth including the ameloblasts. The formation of enamel, termed amelogenesis, occurs through the cytodifferentiation of ameloblasts, ultimately leading to apoptosis and necrosis of these cells with eruption. Therefore, studies regarding enamel matrix formation and bioengineering have been limited. In this study, we establish and characterize two mouse immortalized ameloblast\u2010like cell lines using human papillomavirus 16 (HPV16) E6\/E7 oncogenes for the first time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting and Sample Population<\/jats:title><jats:p>Two mouse EOE dental cell lines (EOE\u20102M and EOE\u20103M).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Isolated EOE primary cells were used to establish clonal cell lines and immortalized using the HPV16 E6\/E7 gene platform. Two established cell lines were characterized by growth rate (Cell Proliferation Assay, MTS), gene (quantitative RT\u2010PCR) and protein (immunocytochemistry) expression profiles, and mineralization potential (in situ alkaline phosphatase (ALP) histochemistry and Xylene Orange staining) in media supplemented with ascorbic acid and \u03b2\u2010glycerophosphate. Gene and protein expression analyses included specific enamel matrix and ameloblast cell markers: Amel, Ambn, Enam, Amtn, ODAM, MMP20, Krt14 and DLX3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both cell lines were maintained in excess of 30 passages, with EOE\u20103M cells proliferating at a slightly higher rate. The cell lines expressed all tested enamel matrix markers and produced a mineralized ECM demonstrating an ameloblast\u2010like profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Two mouse ameloblasts\u2010like immortalized cell lines have been characterized that will be useful tool for studies regarding enamel bioengineering.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ocr.12313","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T07:31:28Z","timestamp":1557473488000},"page":"134-141","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Establishment and characterization of immortalized mouse ameloblast\u2010like cell lines"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0993-864X","authenticated-orcid":false,"given":"Mary","family":"MacDougall","sequence":"first","affiliation":[{"name":"Faculty of Dentistry University of British Columbia Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Mamaeva","sequence":"additional","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham School of Dentistry Birmingham Alabama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changming","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham School of Dentistry Birmingham Alabama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuo","family":"Chen","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio Dental School San Antonio Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411930040050301"},{"key":"e_1_2_7_3_1","first-page":"69","article-title":"Control of ameloblast differentiation","volume":"39","author":"Zeichner\u2010David M","year":"1995","journal-title":"J Dev Biol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2010.03.001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.12.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.053"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409077"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-009-9265-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316886"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01467-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300209"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209509016988"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2010.132"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00277"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.05.144"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3341\/kjo.2006.20.1.47"},{"key":"e_1_2_7_17_1","first-page":"2058","article-title":"Increased expression of 70 kD heat shock protein in cultured primary keratinocytes induced by human papilloma virus 16 E6\/E7 gene","volume":"118","author":"Liao WJ","year":"2005","journal-title":"Chin Med J (Engl)"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2000.108005432.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200290000637"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0909-8836.1999.eos107407.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(92)90110-T"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.285874"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2014.923880"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"}],"container-title":["Orthodontics &amp; 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