{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:14:57Z","timestamp":1764656097152,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T00:00:00Z","timestamp":1608940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Structured Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyse the effect of gain\u2010of\u2010function mutations in the low\u2010density lipoprotein receptor\u2010related protein 5 (Lrp5) on orthodontic tooth movement (OTM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting and Sample Population<\/jats:title><jats:p>A split\u2010mouth study design was utilized. Thirty\u2010two male Lrp5\u2010high bone mass (HBM) knock\u2010in mice including A214V and G171V mutants (n\u00a0=\u00a016\/group) and sixteen C57BL\/6 wild\u2010type (WT) mice were included in the study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A mouse model of OTM was used for mesial movement of the maxillary first molar using a closed\u2010coil nickel titanium (NiTi) spring attached between the molar and the incisors. After 21\u00a0days, the dissected maxillae were scanned for micro\u2010computed tomography (micro\u2010CT) analyses and embedded in methyl methacrylate and paraffin for histological staining and imaging. Histological analyses included immunohistochemistry for sclerostin (Sost), tartrate\u2010resistant acid phosphatase (TRAP) staining for osteoclasts and fluorescent imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>OTM in the A214V and G171V groups was significantly less than the WT group. Bone volume (BV), per cent bone volume (BV\/TV) and trabecular thickness (Tb.Th) were significantly increased in both A241V and G171V animals compared to the WT animals. On the compression side, decreased osteoclast activity was seen in both A214V and G171V groups compared to the WT group. Fluorescent labelling demonstrated that the pattern of bone deposition in the A214V animals was periosteal whereas the G171V animals added bone endocortically.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gain\u2010of\u2010function mutations of Lrp5 decrease orthodontic tooth movement by increasing alveolar bone mass and reducing osteoclast\u2010mediated bone resorption.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ocr.12459","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T20:21:43Z","timestamp":1608236503000},"page":"430-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The effect of overexpression of Lrp5 on orthodontic tooth movement"],"prefix":"10.1111","volume":"24","author":[{"given":"Robert","family":"Holland","sequence":"first","affiliation":[{"name":"Department of Orthodontics and Oral Facial Genetics Indiana University School of Dentistry Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol","family":"Bain","sequence":"additional","affiliation":[{"name":"Histotechnology Indiana University School of Medicine Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rawan S.","family":"Alrasheed","sequence":"additional","affiliation":[{"name":"Indiana University School of Dentistry Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3989-4780","authenticated-orcid":false,"given":"Alexander G.","family":"Robling","sequence":"additional","affiliation":[{"name":"Department of Anatomy &amp; 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