{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T19:52:04Z","timestamp":1778356324490,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T00:00:00Z","timestamp":1667088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Bone remodelling during development and growth is important for craniofacial integrity of offspring. The aim of this study was to investigate the changes in offspring adult skull morphology when the osteoclasts number was altered in utero, using three\u2010dimensional (3D) geometric morphometric analysis (GMA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We altered osteoclasts number in utero via two approaches. First, we generated heterozygous <jats:italic>Ctsk<\/jats:italic><jats:sup>Cre<\/jats:sup>;DTA<jats:sup>fl\/+<\/jats:sup> (diphtheria toxin A) mice. Second, we altered <jats:italic>Ctsk<\/jats:italic> expression in vivo by injecting pregnant wild\u2010type dams at embryonic day (E) 12.5 with in vivo siRNA specific for <jats:italic>Ctsk<\/jats:italic>. Mice were collected at 6\u2009weeks and analysed using geometric morphometric analysis via computed tomography, histomorphometry and gene expression analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Altering osteoclasts number in utero affected the offspring adult skull morphology. Decreased Ctsk and osteoclast numbers were associated with a decrease in cranial vault height and an increase in mandibular body length. Changes in size and shape were observed with an increased number of osteoclasts in Ctsk<jats:sup>Cre<\/jats:sup>;DTA<jats:sup>fl\/+<\/jats:sup> mice, including an increase in cranial vault height, as well as a shortening of mandibular body length and ramus height.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings of this study suggest that modulation of osteoclast numbers during pre\u2010 and post\u2010natal development may be a previously unknown factor in the aetiology of skeletal malocclusions. An improved understanding of the factors affecting bone homeostasis during development and growth may help in the development of future therapies that would target the early intervention of skeletal malocclusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ocr.12614","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T08:01:32Z","timestamp":1665993692000},"page":"338-348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Altering osteoclast numbers using CTSK models in utero affects mice offspring craniofacial morphology"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9208-3381","authenticated-orcid":false,"given":"Mohamed G.","family":"Hassan","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Faculty of Dentistry Assiut University  Assiut Egypt"},{"name":"Program in Craniofacial Biology UCSF  California San Francisco 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