{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T16:21:37Z","timestamp":1773073297889,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T00:00:00Z","timestamp":1680652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Pathological dental root resorption and alveolar bone loss are often detected only after irreversible damage. Biomarkers in the gingival crevicular fluid or saliva could provide a means for early detection; however, such biomarkers have proven elusive. We hypothesize that a multiomic approach might yield reliable diagnostic signatures for root resorption and alveolar bone loss. Previously, we showed that extracellular vesicles (EVs) from osteoclasts and odontoclasts differ in their protein composition. In this study, we investigated the metabolome of EVs from osteoclasts, odontoclasts and clasts (non\u2010resorbing clastic cells).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Mouse haematopoietic precursors were cultured on dentine, bone or plastic, in the presence of recombinant RANKL and CSF\u20101 to trigger differentiation along the clastic line. On Day 7, the cells were fixed and the differentiation state and resorptive status of the clastic cells were confirmed. EVs were isolated from the conditioned media on Day 7 and characterized by nanoparticle tracking and electron microscopy to ensure quality. Global metabolomic profiling was performed using a Thermo Q\u2010Exactive Orbitrap mass spectrometer with a Dionex UHPLC and autosampler.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 978 metabolites in clastic EVs. Of those, 79 are potential biomarkers with Variable Interdependent Parameters scores of 2 or greater. Known metabolites cytidine, isocytosine, thymine, succinate and citrulline were found at statistically higher levels in EVs from odontoclasts compared with osteoclasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that numerous metabolites found in odontoclast EVs differ from those in osteoclast EVs, and thus represent potential biomarkers for root resorption and periodontal tissue destruction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ocr.12658","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:59:50Z","timestamp":1680224390000},"page":"632-641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Metabolomic signatures distinguish extracellular vesicles from osteoclasts and odontoclasts"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-7642","authenticated-orcid":false,"suffix":"Jr","given":"Wellington J.","family":"Rody","sequence":"first","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics University of Pittsburgh, School of Dental Medicine  Pittsburgh Pennsylvania 15261 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan G.","family":"Reuter","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Florida College of Dentistry  Gainesville Florida 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannen E.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Florida College of Dentistry  Gainesville Florida 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina I.","family":"Hammadi","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Florida College of Dentistry  Gainesville Florida 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Macey L.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Florida College of Dentistry  Gainesville Florida 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy G.","family":"Cagmat","sequence":"additional","affiliation":[{"name":"Department of Pathology, Immunology, and Laboratory Medicine University of Florida  Gainesville Florida 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy J.","family":"Garrett","sequence":"additional","affiliation":[{"name":"Department of Pathology, Immunology, and Laboratory Medicine University of Florida  Gainesville Florida 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0844-1965","authenticated-orcid":false,"given":"L. 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