{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T03:39:55Z","timestamp":1792381195051,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100005648","name":"American Association of Orthodontists Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005648","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of the study was to investigate differential gene expression between murine right and left maxilla\u2013mandibular (MxMn) complexes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting and Sample Population<\/jats:title>\n                    <jats:p>Wild\u2010type (WT) C57BL\/6 embryonic (E) day 14.5 (n\u2009=\u20093) and 18.5 (n\u2009=\u20093) murine embryos.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The E14.5 and 18.5 embryos were harvested and hemi\u2010sectioned the MxMn complexes into right and left halves in the mid\u2010sagittal plane. We isolated total RNA using Trizol reagent and further purified using the RNA\u2010easy kit (QIAGEN). We confirmed equal expression of house\u2010keeping genes in right and left halves using RT\u2010PCR and then performed paired\u2010end whole mRNA sequencing in LC Sciences (Houston, TX) followed by differential transcript analyses (&gt;1 or &lt;\u22121 log fold change;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05;\n                      <jats:italic>q<\/jats:italic>\n                      \u2009&lt;\u2009.05; and FPKM &gt;0.5 in 2\/3 samples). The Mouse Genome Informatics and Online Mendelian Inheritance in Man databases as well as gnomAD constraint scores were used to prioritize differentially expressed transcripts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>There were 19 upregulated and 19 downregulated transcripts at E14.5 and 8 upregulated and 17 downregulated transcripts at E18.5 time\u2010points. These differentially expressed transcripts were statistically significant and shown to be associated with craniofacial phenotypes in mouse models. These transcripts also have significant gnomAD constraint scores and are enriched in biological processes critical for embryogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We identified significant differential expression of transcripts between E14.5 and 18.5 murine right and left MxMn complexes. These findings when extrapolated to humans, they may provide a biological basis for facial asymmetry. Further experiments are required to validate these findings in murine models with craniofacial asymmetry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ocr.12660","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T02:57:08Z","timestamp":1681873028000},"page":"39-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptome analyses of murine right and left maxilla\u2013mandibular complex"],"prefix":"10.1111","volume":"26","author":[{"given":"Jacqueline","family":"Crawford","sequence":"first","affiliation":[{"name":"Department of Orthodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Morawski","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Eliason","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Wuebker","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology The University of Iowa  Iowa City Iowa USA"},{"name":"Iowa Institute for Oral Health Research, The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Van\u00a0Otterloo","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology The University of Iowa  Iowa City Iowa USA"},{"name":"Iowa Institute for Oral Health Research, The University of Iowa  Iowa City Iowa USA"},{"name":"Craniofacial Anomalies Research Center The University of Iowa  Iowa City Iowa USA"},{"name":"Department of Periodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huojun","family":"Cao","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, The University of Iowa  Iowa City Iowa USA"},{"name":"Craniofacial Anomalies Research Center The University of Iowa  Iowa City Iowa USA"},{"name":"Department of Endodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Moreno","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"},{"name":"Iowa Institute for Oral Health Research, The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brad","family":"Amendt","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"},{"name":"Department of Anatomy and Cell Biology The University of Iowa  Iowa City Iowa USA"},{"name":"Iowa Institute for Oral Health Research, The University of Iowa  Iowa City Iowa USA"},{"name":"Craniofacial Anomalies Research Center The University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shankar","family":"Rengasamy\u00a0Venugopalan","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, College of Dentistry The University of Iowa  Iowa City Iowa USA"},{"name":"Iowa Institute for Oral Health Research, The University of Iowa  Iowa City Iowa USA"},{"name":"Craniofacial Anomalies Research Center The University of Iowa  Iowa City Iowa 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