{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T13:22:24Z","timestamp":1777036944112,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["1R34DE033595\u201001"],"award-info":[{"award-number":["1R34DE033595\u201001"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Sympathetic\u2010sensory coupling is increasingly implicated in joint homeostasis and disease. Towards the long\u2010term goal of deciphering its role in temporomandibular disorders (TMDs), we characterised the spatial relationships of sympathetic and sensory nerves and their receptors in the mouse temporomandibular joint (TMJ) and trigeminal ganglion (TG), using the knee joint for comparison.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      RNAscope was used for localisation of sympathetic nerve markers tyrosine hydroxylase (\n                      <jats:italic>Th<\/jats:italic>\n                      ) and adrenergic receptor alpha 2a (\n                      <jats:italic>Adr\u03b12a<\/jats:italic>\n                      ); and brain\u2010derived neurotrophic factor (\n                      <jats:italic>Bdnf<\/jats:italic>\n                      ) and its receptor neurotrophic receptor tyrosine kinase 2 (\n                      <jats:italic>Ntrk2<\/jats:italic>\n                      ) that stimulate sympathetic nerve growth. Sensory marker calcitonin gene\u2010related peptide (CGRP) was detected through immunofluorescence.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Th<\/jats:italic>\n                      ,\n                      <jats:italic>Adr\u03b12a<\/jats:italic>\n                      ,\n                      <jats:italic>Bdnf<\/jats:italic>\n                      and\n                      <jats:italic>Ntrk2<\/jats:italic>\n                      were expressed in the TMJ and knee joint tissues, including condylar and disc cartilages, muscles and bone marrow. CGRP+ sensory neurons were abundant in the TG and, to a lesser extent, in TMJ and knee tissues, where they were often located near\n                      <jats:italic>Th<\/jats:italic>\n                      \u2010 or\n                      <jats:italic>Adr\u03b12a<\/jats:italic>\n                      \u2010expressing sympathetic cells. Some CGRP+ neurons in the TG also co\u2010expressed\n                      <jats:italic>Adr\u03b12a<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings reveal the presence of sympathetic and sensory nerves within the TMJ, knee joint and TG, with these nerves often located near each other. Along with the presence of\n                      <jats:italic>Adr\u03b12a<\/jats:italic>\n                      on CGRP+ sensory fibres, this suggests sympathetic\u2010sensory coupling and potential crosstalk in physiologic and pathologic responses. Expression of\n                      <jats:italic>Bdnf<\/jats:italic>\n                      and\n                      <jats:italic>Ntrk2<\/jats:italic>\n                      in the TMJ and knee implicates this neurotrophin signalling in modulating sympathetic activity via neural sprouting. Further studies will clarify the functional contributions of this neurological architecture to tissue homeostasis, disease and pain.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ocr.70031","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T09:15:19Z","timestamp":1757927719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sensory and Sympathetic Nerve Localization in Mouse Temporomandibular Joint and Knee Joint Neuro\u2010Musculoskeletal Tissues"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0077-4300","authenticated-orcid":false,"given":"Qianlin","family":"Ye","sequence":"first","affiliation":[{"name":"Section of Orthodontics University of California  Los Angeles California 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Orthodontics University of California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.11607\/jop.1151"},{"key":"e_1_2_10_3_1","unstructured":"S.Kopp \u201cNeuroendocrine Immune and Local Responses Related to Temporomandibular Disorders 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