{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:50:51Z","timestamp":1781178651812,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471022"],"award-info":[{"award-number":["82471022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471019"],"award-info":[{"award-number":["82471019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301090"],"award-info":[{"award-number":["82301090"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Orthodontic tooth movement (OTM) pain poses a significant clinical challenge, yet its underlying molecular and cellular mechanisms remain incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established a mouse OTM model validated by micro\u2010CT and histology, alongside pain assessments (Mouse Grimace Scale, bite force). Bulk RNA\u2010seq was performed on trigeminal ganglion (TG) tissues, integrated with public\u00a0single\u2010cell RNA\u2010seq data for a more comprehensive view, and key genes were confirmed via qPCR and immunofluorescence. A user\u2010friendly web platform (OTMPTGTA) was developed to enable researchers to explore differentially expressed genes, gene correlations and gene\u2010set analyses without specialised coding skills.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>OTM induced both significant tooth movement and heightened pain, peaking at Day 3. Transcriptomic analyses pinpointed an Atf3+ population of injured TG neurons that showed marked upregulation of xanthine dehydrogenase (Xdh) during peak pain; by Day 14, both gene expression levels and pain scores subsided. QPCR and immunofluorescence confirmed coexpression of Atf3 and Xdh at Day 3, implicating Xdh in nociceptive signalling. The OTMPTGTA platform facilitates further exploration of these and other potential pain\u2010related pathways, offering novel insights for targeted intervention.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Xdh is highly expressed in Atf3+ injured neurons during OTM pain, likely intensifying nociceptive signals through oxidative and inflammatory pathways. These findings highlight Xdh as a promising therapeutic target for alleviating orthodontic pain while preserving effective tooth movement.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ocr.70036","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T12:21:08Z","timestamp":1758889268000},"page":"1048-1058","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Time\u2010Series Transcriptomics Identifies Xanthine Dehydrogenase in Injured Neurons Correlated With Orthodontic Pain"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0707-0928","authenticated-orcid":false,"given":"Xiaoqi","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4056-0080","authenticated-orcid":false,"given":"Xiaoqian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology Affiliated Hospital of Stomatology School of Stomatology Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7873-9516","authenticated-orcid":false,"given":"Waseem","family":"AI\u2010Gumaei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minqi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Bone Metabolism, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; 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