{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T20:32:20Z","timestamp":1767817940986,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Osteocytes express numerous G protein\u2010coupled receptors (GPCRs) and mice lacking the stimulatory subunit of G\u2010protein (Gs\u03b1) in osteocytes (DMP1\u2010Gs\u03b1\n                      <jats:sup>KO<\/jats:sup>\n                      mice) have severe osteopenia. We previously demonstrated that the severe osteopenia in these mice was mediated by increased sclerostin expression, which suppresses osteoblast functions. In this study we analyse both the craniofacial and tooth characteristics of these mutant mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 28, 6\u20138\u2010week\u2010old mice were used (Gs\u03b1 fl\/fl or Control, DMP1\u2010Cre;Gs\u03b1 fl\/fl or DMP1\u2010Gs\u03b1KO). Total bone mineral density (BMD), total bone mineral content (BMC), condyles' BMD and incisor mineralisation were quantitatively assessed by DEXA. Bone microarchitecture of the mandible and full head of mice was quantitatively assessed with high\u2010resolution micro\u2010computed tomography (\u03bcCT). For the condylar region, we assessed total BMD and bone volume fraction (BV\/TV, %). Three linear measurements of the mandible were measured: mandibular length, condyle head length and condyle head width, and samples were assessed by histomorphometric analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>DMP1\u2010Gs\u03b1KO mice have decreased total mandibular BMD, total mandibular BMC, condylar BMD and total tooth mineralisation as assessed by DEXA. \u03bcCT analysis revealed that condylar bone volume and tooth mineralisation were reduced in DMP1\u2010Gs\u03b1KO mice and showed that skull size and specifically the zygomatic bone were larger in control. H&amp;E histological sections of the jaws of DMP1\u2010Gs\u03b1KO mice confirmed the osteopenic phenotype. Tartrate\u2010resistant acid phosphatase (TRAP) staining showed that the number of TRAP\u2010positive osteoclasts was increased in the DMP1\u2010Gs\u03b1KO mice compared to controls, suggesting increased bone resorption.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our studies identified Gs\u03b1 signalling in osteocytes, mature osteoblasts and odontoblasts, as important in maintaining normal bone and tooth homeostasis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ocr.70062","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T07:32:22Z","timestamp":1764142342000},"page":"196-206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gs\u03b1 Deficiency in Osteocytes Alters Craniofacial Growth and Enamel Mineralisation"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4929-8632","authenticated-orcid":false,"given":"Khalid A.","family":"Abalkhail","sequence":"first","affiliation":[{"name":"Department of Translational Dental Medicine, Henry M. Goldman School of Dental Medicine Boston University  Boston Massachusetts USA"},{"name":"Department of Preventive Dental Science College of Dentistry, King Saud bin Abdulaziz University for Health Sciences  Riyadh Saudi Arabia"},{"name":"King Abdullah International Medical Research Center  Riyadh Saudi Arabia"},{"name":"Department of Orthodontics Ministry of National Guard Health Affairs  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6142-7804","authenticated-orcid":false,"given":"Melih","family":"Motro","sequence":"additional","affiliation":[{"name":"Department of Orthodontics &amp; Dentofacial Orthopedics, Henry M. Goldman School of Dental Medicine Boston University  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alpdogan","family":"Kantarci","sequence":"additional","affiliation":[{"name":"Department of Developmental and Surgical Sciences University of Minnesota  Minneapolis Minnesota USA"},{"name":"Department of Immunology ADA Forsyth Institute  Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9853-9837","authenticated-orcid":false,"given":"Paola Divieti","family":"Pajevic","sequence":"additional","affiliation":[{"name":"Department of Translational Dental Medicine, Henry M. Goldman School of Dental Medicine Boston University  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2012-1026"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2016.11.001"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46406"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2270"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.22.2741"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.041210"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-0239"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.06.019"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1665"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.8.7628403"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-437160"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3001"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.628313"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.441360"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-10-0308"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.679753"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2018.09.021"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600404"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1905"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.141"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03428-y"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2007.03.013"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.184754"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.080216"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.081206"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1305224"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.182"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kjms.2018.01.007"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500346200"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/jips.jips_60_17"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212700200"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-22345-2_8"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/adj.12104"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000091385"}],"container-title":["Orthodontics &amp; Craniofacial Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ocr.70062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T19:14:14Z","timestamp":1767813254000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ocr.70062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,25]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/ocr.70062"],"URL":"https:\/\/doi.org\/10.1111\/ocr.70062","archive":["Portico"],"relation":{},"ISSN":["1601-6335","1601-6343"],"issn-type":[{"value":"1601-6335","type":"print"},{"value":"1601-6343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,25]]},"assertion":[{"value":"2025-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}