{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T07:16:07Z","timestamp":1783062967456,"version":"3.54.6"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.083937\/2024\u201000"],"award-info":[{"award-number":["88887.083937\/2024\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.966980\/2024\u201000"],"award-info":[{"award-number":["88887.966980\/2024\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthod Craniofacial Res"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to evaluate the impact of simulated obesity in rats on Receptor Activator of Nuclear Factor \u03baB (RANK), Receptor Activator of Nuclear Factor \u03baB Ligand (RANKL) and Osteoprotegerin (OPG) expression during orthodontic tooth movement (OTM).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Forty male Wistar rats 6\u2009weeks old were allocated into non\u2010obese and obese groups. Obesity was simulated by a high\u2010fat diet for 8\u2009weeks. After, the left maxillary first molar was moved by stretched orthodontic nickel\u2010titanium coiled springs. The animals were euthanized after 7 and 14\u2009days of OTM, and the alveolar bone surrounding the roots of the left and right maxillary first molar was dissected to gene expression by qRT\u2010PCR. TaqMan assays for RANK, RANKL, OPG and GAPDH were used, and the values of gene expression were compared by one\u2010 and two\u2010way ANOVA with Tukey post\u2010test (alpha\u2009=\u20095%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      RANK expression in OTM side at 7\u2009days was statistically different between non\u2010 and obese rats (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), but not at 14\u2009days (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). The RANKL and OPG expression levels on the OTM side did not differ between the groups after 7 or 14\u2009days of OTM.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The RANKL and OPG gene expression is not affected by OTM in simulated obese rats. The impact of obesity on RANK gene expression needs to be further studied.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ocr.70157","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T06:22:52Z","timestamp":1783059772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Simulated Obesity in Animals on\n                    <scp>RANK<\/scp>\n                    \/\n                    <scp>RANKL<\/scp>\n                    \/\n                    <scp>OPG<\/scp>\n                    Pathway During Orthodontic Tooth Movement"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9250-4414","authenticated-orcid":false,"given":"Patr\u00edcia Maria","family":"Monteiro","sequence":"first","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7735-9271","authenticated-orcid":false,"given":"Gabriela Leite","family":"Pedroso","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7854-5559","authenticated-orcid":false,"given":"Maria Cec\u00edlia Gorita","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00edsa Maria Vellasco Queiroz","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Gollino","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-8715","authenticated-orcid":false,"given":"Mirian Aiko Nakane","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7791-9144","authenticated-orcid":false,"given":"Maria Bernadete Sasso","family":"Stuani","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo (USP)  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"e_1_2_14_2_1","volume-title":"Obesity and Overweight","author":"World Health Organization"},{"key":"e_1_2_14_3_1","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/nature05485","article-title":"Inflammation and Metabolic Disorders","volume":"444","author":"Hotamisligil G.","year":"2006","journal-title":"Nature"},{"key":"e_1_2_14_4_1","doi-asserted-by":"crossref","DOI":"10.4061\/2011\/529061","article-title":"Inflammatory Concepts of Obesity","volume":"2011","author":"Rocha V. 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