{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:13:04Z","timestamp":1789377184677,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T00:00:00Z","timestamp":1363564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (<jats:styled-content style=\"fixed-case\">iSHFD<\/jats:styled-content>) and to evaluate the influence of fibroblastic growth factor\u20102 (FGF\u20102) on the regenerative potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF\u20102 (20\u00a0ng\u00a0ml<jats:sup>\u22121<\/jats:sup>) during <jats:italic>ex vivo<\/jats:italic> expansion. Isolated stem cells expanded in FGF\u20102 were characterized using a colony\u2010forming assay, proliferation, migration, <jats:italic>in vitro<\/jats:italic> differentiation, <jats:italic>in vivo<\/jats:italic> ectopic transplantation assay, and gene expression profiling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF\u20102 applied to <jats:styled-content style=\"fixed-case\">iSHFD<\/jats:styled-content> during expansion enhanced the colony\u2010forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential <jats:italic>in vitro<\/jats:italic>. However, the ectopic transplantation of <jats:styled-content style=\"fixed-case\">iSHFD<\/jats:styled-content>\/FGF\u20102 <jats:italic>in vivo<\/jats:italic> increased the formation of dentin\u2010like material.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FGF<\/jats:styled-content>\u20102 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12089","type":"journal-article","created":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T21:07:01Z","timestamp":1361480821000},"page":"191-204","source":"Crossref","is-referenced-by-count":54,"title":["Treatment of <scp>FGF<\/scp>\u20102 on stem cells from inflamed dental pulp tissue from human deciduous 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