{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:59:18Z","timestamp":1777381158382,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,11,29]],"date-time":"2013-11-29T00:00:00Z","timestamp":1385683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Objective<\/jats:title>Our poor understanding of how inflammatory mediators can affect osteoblast behavior led us to investigate the tumor necrosis factor (TNF<\/jats:styled-content>)\u03b1<\/jats:italic>\u2010induced focal adhesion kinase (FAK<\/jats:styled-content>) and S<\/jats:styled-content>rc phosphorylation.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>MC<\/jats:styled-content>3T3\u2010E1 pre\u2010osteoblast cells were harvested at specific time points after either TNF<\/jats:styled-content>\u03b1<\/jats:italic> treatment or RAW<\/jats:styled-content>267 stimulated conditioned medium, and thereafter cell extracts were prepared for I<\/jats:styled-content>mmunoblotting assay. ELISA<\/jats:styled-content> detected TNF<\/jats:styled-content>\u03b1<\/jats:italic> content at conditioned medium. T<\/jats:styled-content>umor necrosis factor\u2010\u03b1<\/jats:italic>\u2010neutralizing antibodies also were used.<\/jats:p><\/jats:sec>Results<\/jats:title>It was possible to show that TNF<\/jats:styled-content>\u03b1<\/jats:italic> provokes attenuation at Y<\/jats:styled-content>\u2010phosphorylation of both FAK<\/jats:styled-content> (at Y<\/jats:styled-content>397<\/jats:sub>) and S<\/jats:styled-content>rc (at Y<\/jats:styled-content>416<\/jats:sub>) proteins (P<\/jats:italic>\u00a0<\u00a00.05), suggesting a decrease in their activities. The very similar profile was observed when osteoblasts were incubated with conditioned medium from lipopolysaccharide (LPS<\/jats:styled-content>)\u2010stimulated macrophages, it being significantly different than control (FAK<\/jats:styled-content> and S<\/jats:styled-content>rc, P<\/jats:italic>\u00a0<\u00a00.05). Nevertheless, in order to validate these findings, we decided to pre\u2010incubate osteoblasts with anti\u2010TNF<\/jats:styled-content>\u03b1<\/jats:italic> neutralizing antibody (2\u00a0\u03bc<\/jats:italic>g\u00a0ml\u22121<\/jats:sup>) prior exposing to conditioned medium. Importantly, our results revealed that there was a diminution on those conditioned medium effects when the same biological parameters were evaluated (P<\/jats:italic>\u00a0<\u00a00.05). Moreover, we also showed that TNF<\/jats:styled-content>\u03b1<\/jats:italic> impairs osteoblast adhesion, suggesting an interesting role on osteoblast performance.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Altogether, these results suggest that LPS<\/jats:styled-content>\u2010stimulated macrophage mediators attenuate both FAK<\/jats:styled-content> and S<\/jats:styled-content>rc activations in osteoblast, suggesting a novel role for TNF<\/jats:styled-content>\u03b1<\/jats:italic> on osteoblast performance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12202","type":"journal-article","created":{"date-parts":[[2013,10,27]],"date-time":"2013-10-27T11:59:55Z","timestamp":1382875195000},"page":"780-786","source":"Crossref","is-referenced-by-count":22,"title":["TNF<\/scp>\u03b1<\/i> contributes for attenuating both Y<\/scp>397<\/sub>FAK<\/scp> and Y<\/scp>416<\/sub>S<\/scp>rc phosphorylations in osteoblasts"],"prefix":"10.1111","volume":"20","author":[{"given":"ADM","family":"Cavagis","sequence":"first","affiliation":[{"name":"Federal University of S\u00e3o Carlos (UFSCar) Sorocaba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ER","family":"Takamori","sequence":"additional","affiliation":[{"name":"Laboratorio Multidisciplinar em Pesquisa Odontol\u00f3gica (LAMP) Odontologia Escola de Ci\u00eancias da Sa\u00fade Universidade do Grande Rio (UNIGRANRIO) Duque de Caxias Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JM","family":"Granjeiro","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Metrologia Normaliza\u00e7\u00e3o e Qualidade Industrial \u2013 INMETRO Diretoria de Programas \u2013 DIPRO \/ Bioengenharia Xer\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RC","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bauru Dental School FOB\u2010USP Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CV","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Biology State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MP","family":"Peppelenbosch","sequence":"additional","affiliation":[{"name":"Kinome Profiling Unit Department of Cell Biology University Medical Center Groningen University of Groningen Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WF","family":"Zambuzzi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bioensaios e Din\u00e2mica Celular Department Chemistry and Biochemistry Biosciences Institute UNESP campus Botucatu Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300903"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.43.2.187"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.49.6.610"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.4.1109"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"20567","DOI":"10.1016\/S0021-9258(17)32031-8","article-title":"A transmembrane\u2010anchored chimeric focal adhesion kinase is constitutively activated and phosphorylated at tyrosine residues identical to pp125FAK","volume":"269","author":"Chan PY","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.04.013"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24622"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510376402"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.11.7739"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106339200"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.06.002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90094-2"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(96)00181-0"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.2.481"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.4.1289-1297.1994"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3287-3296.1995"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2005.10.054"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22479"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-530-9_15"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.5.2571"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(01)02152-3"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.8.3511"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5442.1172"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2007.08.024"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.10.013"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22236"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000185506"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.01.002"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fodi.12202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.12202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T05:29:04Z","timestamp":1696224544000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.12202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,29]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1111\/odi.12202"],"URL":"https:\/\/doi.org\/10.1111\/odi.12202","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,29]]}}}