{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:28:44Z","timestamp":1789972124463,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,29]],"date-time":"2013-11-29T00:00:00Z","timestamp":1385683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"crossref","award":["R21 DE018717"],"award-info":[{"award-number":["R21 DE018717"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study was to identify differentially expressed salivary proteins in bisphosphonate\u2010related osteonecrosis of the jaw (<jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content>) patients that could serve as biomarkers for <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Whole saliva obtained from 20 <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> patients and 20 controls were pooled within groups. The samples were analyzed using i<jats:styled-content style=\"fixed-case\">TRAQ<\/jats:styled-content>\u2010labeled two\u2010dimensional liquid chromatography\u2013tandem mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 1340 proteins were identified. Of these, biomarker candidates were selected based on <jats:italic>P<\/jats:italic>\u2010value (&lt;0.001), changes in protein expression (\u22651.5\u2010fold increase or decrease), and unique peptides identified (\u22652). Three comparisons made between <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> and control patients identified 200 proteins to be differentially expressed in <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> patients. A majority of these proteins were predicted to have a role in drug metabolism and immunological and dermatological diseases. Of all the differentially expressed proteins, we selected metalloproteinase\u20109 and desmoplakin for further validation. Immunoassays confirmed increased expression of metalloproteinase\u20109 in individual saliva (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.048) and serum samples (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.05) of <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> patients. Desmoplakin was undetectable in saliva. However, desmoplakin levels tended to be lower in <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> serum than controls (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.157).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Multiple pathological reactions are involved in <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> development. One or more proteins identified by this study may prove to be useful biomarkers for <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> diagnosis. The role of metalloproteinase\u20109 and desmoplakin in <jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> requires further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12204","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T15:08:33Z","timestamp":1383232113000},"page":"46-56","source":"Crossref","is-referenced-by-count":24,"title":["Salivary proteomics in bisphosphonate\u2010related osteonecrosis of the jaw"],"prefix":"10.1111","volume":"21","author":[{"given":"V","family":"Thumbigere\u2010Math","sequence":"first","affiliation":[{"name":"Division of Periodontology University of Minnesota School of Dentistry Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BS","family":"Michalowicz","sequence":"additional","affiliation":[{"name":"Division of Periodontology University of Minnesota School of Dentistry Minneapolis MN 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