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The rats were fixed in weeks 1, 2, 4 and 8, and the calvaria were resected and examined with microcomputed tomography, then frozen and sectioned for histology and analysed with energy\u2010dispersive X\u2010ray spectroscopy (<jats:styled-content style=\"fixed-case\">EDX<\/jats:styled-content>). Parietal bone of 12\u2010week\u2010old control rats was processed similarly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mineral density of healing bone increased with time. The healing bone became thicker and denser with time in histology. The distributions of Ca and P expanded over the bone matrix, whereas that of C became localised and complemented that of C and P. The Ca\/P concentration ratio increased, whereas the C\/Ca and C\/P ratios decreased in the healing bone matrix.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Healing bone is immaturely calcified initially and proceeds calcification gradually, that is, as the bone volume increases, mineral increases in density and matures in quality, while organic components decrease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12212","type":"journal-article","created":{"date-parts":[[2013,11,26]],"date-time":"2013-11-26T05:21:56Z","timestamp":1385443316000},"page":"74-82","source":"Crossref","is-referenced-by-count":24,"title":["Calcification during bone healing in a standardised rat calvarial defect assessed by micro\u2010<scp>CT<\/scp>and<scp>SEM<\/scp>\u2010<scp>EDX<\/scp>"],"prefix":"10.1111","volume":"21","author":[{"given":"H","family":"Okata","sequence":"first","affiliation":[{"name":"Division of Periodontology and Endodontology Tohoku University Graduate School of 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