{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:21:10Z","timestamp":1770747670159,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Accumulating evidence indicates that aberrant <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation is closely related to oral carcinogenesis, and it has been shown that methylation changes might be used as prognostic biomarker in oral squamous cell carcinoma. Oral lichenoid disease (<jats:styled-content style=\"fixed-case\">OLD<\/jats:styled-content>) is the most common oral potentially malignant disorder in our region. The aim of this study was to perform the first wide <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation study in <jats:styled-content style=\"fixed-case\">OLD<\/jats:styled-content> in order to investigate the relevance of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation changes in this premalignant disorder.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Two different Illumina microarray platforms, namely the GoldenGate Cancer Panel I and the HumanMethylation27 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> Analysis BeadChip, were utilized in the discovery phase to interrogate the methylation profile of 59 <jats:styled-content style=\"fixed-case\">OLD<\/jats:styled-content> cases and 9 healthy individuals. Top\u2010ranked genes were further validated by pyrosequencing in a second sample set consisting of 160 <jats:styled-content style=\"fixed-case\">OLD<\/jats:styled-content> and 65 controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results show that the frequency of aberrant <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation is rare in <jats:styled-content style=\"fixed-case\">OLD<\/jats:styled-content>, and this finding was further corroborated by pyrosequencing in the biological validation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings reinforce the notion that molecular alterations associated with oral carcinogenesis do not seem to be common events in <jats:styled-content style=\"fixed-case\">OLD<\/jats:styled-content>, which in turn might explain the low rate of malignization of this disorder.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12243","type":"journal-article","created":{"date-parts":[[2014,4,12]],"date-time":"2014-04-12T05:26:47Z","timestamp":1397280407000},"page":"821-826","source":"Crossref","is-referenced-by-count":8,"title":["Global <scp>DNA<\/scp> methylation: uncommon event in oral lichenoid disease"],"prefix":"10.1111","volume":"20","author":[{"given":"NG","family":"Bediaga","sequence":"first","affiliation":[{"name":"Oral Medicine &amp; Pathology Department of Stomatology II, UFI 11\/25 University of the Basque Country (UPV\/EHU) Leioa Spain"},{"name":"BIOMICs Research Group University of the Basque Country (UPV\/EHU) Vitoria\u2010Gasteiz Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X","family":"Marichalar\u2010Mendia","sequence":"additional","affiliation":[{"name":"Oral Medicine &amp; Pathology Department of Stomatology II, UFI 11\/25 University of the Basque Country (UPV\/EHU) Leioa Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JM","family":"Aguirre\u2010Urizar","sequence":"additional","affiliation":[{"name":"Oral Medicine &amp; Pathology Department of Stomatology II, UFI 11\/25 University of the Basque Country (UPV\/EHU) Leioa Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Calvo","sequence":"additional","affiliation":[{"name":"Intelligent Systems Group University of the Basque Country (UPV\/EHU) Donostia \u2013 San Sebasti\u00e1n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MA","family":"Echebarria\u2010Goicouria","sequence":"additional","affiliation":[{"name":"Oral Medicine &amp; 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