{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:33:43Z","timestamp":1791405223571,"version":"4.3.3"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T00:00:00Z","timestamp":1429833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22792085"],"award-info":[{"award-number":["22792085"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>A large number of individuals have halitosis. The total amount of volatile sulfur compounds, which are the main cause of halitosis, has been correlated with periodontitis following bacterial infection. In this study, <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>Pg<\/jats:italic>), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>, and the ability of isolated <jats:italic>Pg<\/jats:italic> to produce methyl mercaptan (<jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>) was determined to clarify the relationship between halitosis and <jats:italic>Pg<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> concentrations were measured in patients using Oral Chroma. The production of <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> by <jats:italic>Pg<\/jats:italic> standard and clinical strains was also measured <jats:italic>in\u00a0vitro<\/jats:italic>. Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was performed to compare the expression of <jats:italic>mgl <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> (which encoded <jats:sc>l<\/jats:sc>\u2010methionine\u2010a\u2010deamino\u2010g\u2010mercaptomethane\u2010lyase) among the <jats:italic>Pg<\/jats:italic> strains. The production of <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> and the expression of <jats:italic>mgl <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> were also determined to assess the effects of oriental medicine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The production of <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> and the expression of <jats:italic>mgl <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> strongly correlated with each other in the presence of <jats:sc>l<\/jats:sc>\u2010methionine. The expression of <jats:italic>mgl <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> by <jats:italic>Pg<\/jats:italic> W83 was strongly inhibited by <jats:italic>magnoliaceae<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The production of <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> was correlated with the expression of <jats:italic>mgl<\/jats:italic>. Furthermore, the oriental medicine, <jats:italic>magnoliaceae<\/jats:italic>, may represent a potential treatment for halitosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12326","type":"journal-article","created":{"date-parts":[[2015,2,23]],"date-time":"2015-02-23T05:42:58Z","timestamp":1424670178000},"page":"626-633","source":"Crossref","is-referenced-by-count":11,"title":["The differential expression of <i>mgl <\/i><scp>mRNA<\/scp> by <i>Porphyromonas gingivalis<\/i> affects the production of methyl mercaptan"],"prefix":"10.1111","volume":"21","author":[{"given":"K","family":"Ouhara","sequence":"first","affiliation":[{"name":"Division of Applied Life Sciences Department of Periodontal Medicine Institute of Biomedical &amp; Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences Department of Periodontal Medicine 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