{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T07:18:20Z","timestamp":1695799100165},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T00:00:00Z","timestamp":1452470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["23592735","21791805"],"award-info":[{"award-number":["23592735","21791805"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005623","name":"University of Tokushima","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005623","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005981","name":"Direktorat Jenderal Pendidikan Tinggi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005981","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005623","name":"University of Tokushima","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Disruption of the third zinc finger domain of specificity protein 6 (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6) presents an enamel\u2010specific defect in a rat model of amelogenesis imperfecta (<jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content> rats). To understand the molecular basis of amelogenesis imperfecta caused by the <jats:italic>Sp6<\/jats:italic> mutation, we established and characterized <jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content>\u2010derived rat dental epithelial (<jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content>) cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content> cell clones were isolated from the mandibular incisors of <jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content> rats, and amelogenesis\u2010related gene expression was analyzed by\u00a0reverse transcription polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Localization of wild\u2010type <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6 (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) and mutant\u2010type <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6 (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content>) was analyzed by immunocytochemistry. <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6 transcriptional activity was monitored by <jats:italic>rho\u2010associated protein kinase 1 (Rock1)<\/jats:italic> promoter activity with its specific binding to the promoter region in dental (G5 and <jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content>) and non\u2010dental (<jats:styled-content style=\"fixed-case\">COS<\/jats:styled-content>\u20107) epithelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Isolated <jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content> cells were varied in morphology and gene expression. Both <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content> were mainly detected in nuclei. The promoter analysis revealed that <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content> enhanced <jats:italic>Rock1<\/jats:italic> promoter activity in G5 cells but that enhancement by <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content> was weaker, whereas no enhancement was observed in the <jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">COS<\/jats:styled-content>\u20107 cells, even though <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6<jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content> bound to the promoter in all instances.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ARE<\/jats:styled-content> cell clones can provide a useful <jats:italic>in\u00a0vitro<\/jats:italic> model to study the mechanism of <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>6\u2010mediated amelogenesis imperfecta.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12396","type":"journal-article","created":{"date-parts":[[2015,11,19]],"date-time":"2015-11-19T08:00:43Z","timestamp":1447920043000},"page":"132-139","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and characterization of dental epithelial cells\u00a0derived from amelogenesis imperfecta rat"],"prefix":"10.1111","volume":"22","author":[{"given":"A","family":"Adiningrat","sequence":"first","affiliation":[{"name":"Graduate School of Oral Sciences Tokushima University Tokushima 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