{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:11:38Z","timestamp":1777392698232,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T00:00:00Z","timestamp":1455148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN 327846\u201011"],"award-info":[{"award-number":["RGPIN 327846\u201011"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>A challenge in engineering tissues is to supply parenchymal cells with suitable scaffolds which ideally reproduce the extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>). This study tested the hypothesis of preserving the \u2018residual connective tissue\u2019 remaining after mechanical and enzymatic release of cells from human submandibular gland biopsies (that we named \u2018natural ExtraCellular Matrix scaffolds\u2019, <jats:styled-content style=\"fixed-case\">nECM<\/jats:styled-content>sc) to be used as recycled natural scaffolds. The objective was to test whether <jats:styled-content style=\"fixed-case\">nECM<\/jats:styled-content>sc and native salivary tissue were comparable morphologically, in <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> proteins composition, and in cell seeding efficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Following cell isolation procedures, <jats:styled-content style=\"fixed-case\">nECM<\/jats:styled-content>sc were kept, either fresh or frozen (sectioned into 12\u2010<jats:italic>\u03bc<\/jats:italic>m\u2010thick slices), and examined with high\u2010resolution electron microscopy (<jats:styled-content style=\"fixed-case\">HRSEM<\/jats:styled-content>) for its three\u2010dimensional structure, and with picrosirius red staining and immunogold staining for <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> protein composition and distribution, respectively. <jats:styled-content style=\"fixed-case\">nECM<\/jats:styled-content>sc were seeded with human epithelial cells and fibroblasts to assess cell attachment and proliferation in short\u2010term experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under <jats:styled-content style=\"fixed-case\">HRSEM<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> nECM<\/jats:styled-content>sc had comparable fiber arrangement to original glands. Histochemical and immunogold\u2010labeling examinations revealed the presence of collagen types I, <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>. Seeded epithelial cells and fibroblasts attached, proliferated (14\u201355%), and were alive (86\u201399%) after 4\u20138\u00a0days of culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">nECM<\/jats:styled-content>sc retained native <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> proteins and maintained their distribution. Seeded cells remained viable on <jats:styled-content style=\"fixed-case\">nECM<\/jats:styled-content>sc.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12444","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T15:22:17Z","timestamp":1453303337000},"page":"313-323","source":"Crossref","is-referenced-by-count":19,"title":["Natural extracellular matrix scaffolds recycled from human salivary digests: a morphometric study"],"prefix":"10.1111","volume":"22","author":[{"given":"MA","family":"Lilliu","sequence":"first","affiliation":[{"name":"Faculty of Dentistry McGill University Montreal QC Canada"},{"name":"Department of Biomedical Sciences University of Cagliari, Cittadella Universitaria di Monserrato Monserrato Cagliari Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YJ","family":"Seo","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry McGill University Montreal QC 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