{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:30:02Z","timestamp":1770445802095,"version":"3.49.0"},"reference-count":57,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,7,4]],"date-time":"2016-07-04T00:00:00Z","timestamp":1467590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Characterize the cell profile and immunostaining of proinflammatory markers in an experimental model of bisphosphonate\u2010related osteonecrosis of the jaw (<jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Male Wistar rats (<jats:italic>n<\/jats:italic>\u00a0=\u00a06\u20137) were treated chronically with saline solution or zoledronic acid (<jats:styled-content style=\"fixed-case\">ZA<\/jats:styled-content>) at 0.04, 0.20, and 1.00\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup> (1.4\u00a0\u00d7\u00a010<jats:sup>\u22127<\/jats:sup>, 6.9\u00a0\u00d7\u00a010<jats:sup>\u22126<\/jats:sup>, and 3.4\u00a0\u00d7\u00a010<jats:sup>\u22125<\/jats:sup>\u00a0mol\u00a0kg<jats:sup>\u22121<\/jats:sup>), and subsequently, the first left inferior molar was extracted. Were performed counting of viable and empty osteocyte lacunae, viable and apoptotic osteoclasts, polymorphonuclear neutrophil, mast cells (toluidine blue), and the positive presence cells for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68, tumor necrosis factor\u2010alpha (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1), <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> (interleukin)\u20101\u03b2, inducible nitric oxide synthase (<jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content>), nuclear factor\u2010kappa B (<jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">kB<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201018 binding protein (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201018\u00a0bp).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> was showed in <jats:styled-content style=\"fixed-case\">ZA<\/jats:styled-content> treated with 0.20 and 1.00\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup>. There is a dose dependent increase in percentage of empty osteocyte lacunae (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and apoptotic osteoclasts (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), counting of total osteoclasts (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003), polymorphonuclear neutrophil cells (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.009), cytoplasmic\u2010positive cells of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001), <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001), <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">kB<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.006), and nuclear\u2010positive cells of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">kB<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.011). Consequently, there is no difference in mast cells (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.957), and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201018\u00a0bp immunostaining decreases dose dependently (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.005).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BRONJ<\/jats:styled-content> is characterized by increases in immunostaining for proinflammatory markers and <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">kB<\/jats:styled-content> and inversely associated with cells exhibiting <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201018\u00a0bp.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12513","type":"journal-article","created":{"date-parts":[[2016,5,27]],"date-time":"2016-05-27T14:46:51Z","timestamp":1464360411000},"page":"649-657","source":"Crossref","is-referenced-by-count":36,"title":["Immune cellular profile of bisphosphonate\u2010related osteonecrosis of the jaw"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1513-9027","authenticated-orcid":false,"given":"PG","family":"de Barros Silva","sequence":"first","affiliation":[{"name":"Department of Dental Clinic Division of Oral Pathology Faculty of Pharmacy Dentistry and Nursing Federal University of Ceara Fortaleza Ceara Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CC","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Dental Clinic Division of Oral Pathology Faculty of Pharmacy Dentistry and Nursing Federal 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