{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:36:08Z","timestamp":1785386168665,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81170967"],"award-info":[{"award-number":["81170967"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570975"],"award-info":[{"award-number":["81570975"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to optimize the culture system of keratinocytes obtained from patients with oral lichen planus (<jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>) and verify whether this model could simulate the local inflammatory environment of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Keratinocytes were isolated from 48 patients with <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> and cultured <jats:italic>in\u00a0vitro<\/jats:italic>. The ultrastructure of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> keratinocytes was observed via electron microscopy. The expression of pancytokeratin and vimentin was determined by immunohistochemistry, and the proliferation of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> keratinocytes was measured by <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 assay. Immunofluorescence staining was used to detect <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4 and <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:italic>\u03ba<\/jats:italic>B p65 expression, and the levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:italic>\u03b2<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>\u20106, and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010<jats:italic>\u03b1<\/jats:italic> in the supernatant were measured by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When seeded in plates precoated with recombinant human type\u20101 collagen, keratinocytes isolated from patients who received systemic antifungal treatment and were younger than 40\u00a0years were more successful to be cultured <jats:italic>in\u00a0vitro<\/jats:italic>. Characteristic pancytokeratin was expressed in almost all <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> keratinocytes. Compared with normal oral keratinocytes, <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> keratinocytes demonstrated higher levels of <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4\/<jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:italic>\u03ba<\/jats:italic>B p65 and inflammatory cytokines, including <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:italic>\u03b2<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>\u20106, and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010<jats:italic>\u03b1<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We successfully optimized the culture system of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> keratinocytes,which mimicked the local inflammatory environment of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> and may be used as a cell model of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12599","type":"journal-article","created":{"date-parts":[[2016,10,20]],"date-time":"2016-10-20T15:02:41Z","timestamp":1476975761000},"page":"225-232","source":"Crossref","is-referenced-by-count":17,"title":["<i>In vitro<\/i> cell culture system optimization of keratinocytes from oral lichen planus (<scp>OLP<\/scp>) patients"],"prefix":"10.1111","volume":"23","author":[{"given":"T","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Oral Medicine Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Department of Endodontics and Oral Medicine Nantong Stomatological Hospital Nantong 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