{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T23:19:24Z","timestamp":1772752764106,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,23]],"date-time":"2016-12-23T00:00:00Z","timestamp":1482451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyze the effects of <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 on proliferation, invasion, and metastasis in tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 knockdown was performed in <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>15 cell lines, and functional assay was applied to observe the effects on cell migration and invasion. Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, Western blotting, and immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) were also used to measure the expression of <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 expression was decreased after lentivirus infection. Functional assay showed that silence of <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 can inhibit the proliferation of <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>15 cells and arrest the cells in G1\/S phase. Moreover, knockdown of <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 enhanced apoptosis of <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>15 cells. Wound\u2010healing assay and transwell assay indicated that knockdown of <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 significantly inhibited migration and invasion ability of <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>15 cells. Expression detection suggested that <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 may be involved in the metastasis of <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>15 cells by activating Twist family expression and inducing epithelial\u2013mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>) process. <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> analysis showed that <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 and vimentin were up\u2010regulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues, while E\u2010cadherin was down\u2010regulated. Clinicopathological analysis indicated that expression of <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2, E\u2010cadherin, and Vimentin were associated with recurrence of patients with <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings demonstrated that <jats:styled-content style=\"fixed-case\">HMGA<\/jats:styled-content>2 may promote malignant transformation of <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> through <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> process and may be an independent prognosis biomarker for <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12608","type":"journal-article","created":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T13:49:17Z","timestamp":1478180957000},"page":"255-264","source":"Crossref","is-referenced-by-count":18,"title":["<scp>HMGA<\/scp>2 is associated with the aggressiveness of tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"23","author":[{"given":"H","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oncology The Second Xiangya Hospital Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Xiangya Stomatological Hospital &amp; School of Stomatology Central South University Changsha 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