{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:00:18Z","timestamp":1777496418001,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Fourier Transform Infrared microspectroscopy let characterize the macromolecular composition and distribution of tissues and cells, by studying the interaction between infrared radiation and matter. Therefore, we hypothesize to exploit this analytical tool in the analysis of inflamed pulps, to detect the different biochemical features related to various degrees of inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> maps of 13 irreversible and 12 hyperplastic pulpitis, together with 10 normal pulps, were acquired, compared with histological findings and submitted to multivariate (<jats:styled-content style=\"fixed-case\">HCA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PCA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> SIMCA<\/jats:styled-content>) and statistical (one\u2010way <jats:styled-content style=\"fixed-case\">ANOVA<\/jats:styled-content>) analysis. The fit of convoluted bands let calculate meaningful band area ratios (means\u00a0\u00b1\u00a0s.d., <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The infrared imaging analysis pin\u2010pointed higher amounts of water and lower quantities of type I collagen in all inflamed pulps. Specific vibrational markers were defined for irreversible pulpitis (Lipids\/Total Biomass, Ph<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\/Total Biomass, <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>2<\/jats:sub>\/<jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub>, and Ty\/<jats:styled-content style=\"fixed-case\">AII<\/jats:styled-content>) and hyperplastic ones (<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>\/Total Biomass, Collagen\/Total Biomass, and <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub> Collagen\/Total Biomass).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study confirmed that <jats:styled-content style=\"fixed-case\">FTIR<\/jats:styled-content> microspectroscopy let discriminate tissues\u2019 biological features. The infrared imaging analysis evidenced, in inflamed pulps, alterations in tissues\u2019 structure and composition. Changes in lipid metabolism, increasing amounts of tyrosine, and the occurrence of phosphorylative processes were highlighted in irreversible pulpitis, while high amounts of water and low quantities of type I collagen were detected in hyperplastic samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12635","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T15:23:21Z","timestamp":1483975401000},"page":"484-491","source":"Crossref","is-referenced-by-count":21,"title":["Fourier Transform Infrared Imaging analysis of dental pulp inflammatory diseases"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0503-5870","authenticated-orcid":false,"given":"E","family":"Giorgini","sequence":"first","affiliation":[{"name":"Department of Life and Environmental Sciences Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Sabbatini","sequence":"additional","affiliation":[{"name":"Department of Materials, Environmental Sciences and Urban Planning Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Conti","sequence":"additional","affiliation":[{"name":"Department of Materials, Environmental Sciences and Urban Planning Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Rubini","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Rocchetti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Fioroni","sequence":"additional","affiliation":[{"name":"Department of Odontostomatologic and Specialized Clinical Sciences Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Mem\u00e8","sequence":"additional","affiliation":[{"name":"Department of Odontostomatologic and Specialized Clinical Sciences Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Orilisi","sequence":"additional","affiliation":[{"name":"Department of Odontostomatologic and Specialized Clinical Sciences Polytechnic University of Marche Ancona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,22]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"ZD23","article-title":"Management of chronic hyperplastic pulpitis in mandibular 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