{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T20:00:30Z","timestamp":1762113630857,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T00:00:00Z","timestamp":1490313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2017,7]]},"abstract":"Objectives<\/jats:title>Somatic mutations of the PIK<\/jats:styled-content>3CA<\/jats:styled-content><\/jats:italic>, CASP<\/jats:styled-content>8,<\/jats:italic> and NOTCH<\/jats:styled-content>1<\/jats:italic> have been frequently detected in various human cancers. Our study aimed to analyze the mutational status of these genes in South Indian oral cancers.<\/jats:p><\/jats:sec>Subjects and methods<\/jats:title>We performed mutational analysis of the PIK<\/jats:styled-content>3CA<\/jats:styled-content><\/jats:italic> (exons 9 and 20), CASP<\/jats:styled-content>8<\/jats:italic> (exon 9), and NOTCH<\/jats:styled-content>1<\/jats:italic> (exons 5, 6, 7, 8, and 9) genes in 96, 48, and 44 oral cancer samples, respectively. All the specified exons were PCR<\/jats:styled-content> (polymerase chain reaction)\u2010amplified and directly sequenced by Sanger sequencing.<\/jats:p><\/jats:sec>Results<\/jats:title>PIK<\/jats:styled-content>3CA<\/jats:styled-content><\/jats:italic> gene mutations were not found; however, a synonymous single nucleotide polymorphism (SNP<\/jats:styled-content>) [rs17849079] was observed frequently [35\/96 (36.4%)] in oral cancer samples. Further, no mutations were detected in the CASP<\/jats:styled-content>8<\/jats:italic> gene, but observed a frequent [32\/48 (66.6%)] SNP<\/jats:styled-content> [rs1045487] in the oral cancer samples. We did not detect any mutation in the NOTCH<\/jats:styled-content>1<\/jats:italic> gene (exons 5, 6, 7, 8, and 9) in all the [0\/44] analyzed oral cancer samples.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>This is the first study that reports the status of the PIK<\/jats:styled-content>3CA<\/jats:styled-content><\/jats:italic>, CASP<\/jats:styled-content>8,<\/jats:italic> and NOTCH<\/jats:styled-content>1<\/jats:italic> mutations in South Indian oral cancer samples. Our study suggests that either mutations in these genes are uncommon in South Indian oral cancer samples or likely other genes in this pathway might be mutated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12655","type":"journal-article","created":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T12:26:56Z","timestamp":1486643216000},"page":"669-673","source":"Crossref","is-referenced-by-count":7,"title":["Absence of the frequently reported PIK<\/scp>3CA<\/scp><\/i>,CASP<\/scp>8,<\/i> and NOTCH<\/scp>1<\/i> mutations in South Indian oral cancers"],"prefix":"10.1111","volume":"23","author":[{"given":"G","family":"Arunkumar","sequence":"first","affiliation":[{"name":"Department of Genetics Dr. ALM PG Institute of Basic Medical Sciences University of Madras Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AK","family":"Murugan","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology King Faisal Specialist Hospital and Research Centre Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Nagarajan","sequence":"additional","affiliation":[{"name":"Department of Genetics Dr. ALM PG Institute of Basic Medical Sciences University of Madras Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Ajay","sequence":"additional","affiliation":[{"name":"Center for Oncology Royapettah Government Hospital and Kilpauk Medical College Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Rajaraman","sequence":"additional","affiliation":[{"name":"Center for Oncology Royapettah Government Hospital and Kilpauk Medical College Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1337-9008","authenticated-orcid":false,"given":"AK","family":"Munirajan","sequence":"additional","affiliation":[{"name":"Department of Genetics Dr. ALM PG Institute of Basic Medical 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