{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:42:47Z","timestamp":1768315367050,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP\u2010119585"],"award-info":[{"award-number":["MOP\u2010119585"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,3]]},"abstract":"Objective<\/jats:title>Bone marrow cell extract (BMCE<\/jats:styled-content>) was previously reported to restore salivary gland hypofunction caused by irradiation injury. Proteins were shown to be the main active factors in BMCE<\/jats:styled-content>. However, BMCE<\/jats:styled-content> therapy requires multiple injections and protein denaturation is a concern during BMCE<\/jats:styled-content> storage. This study aimed to preserve, by lyophilization (freeze\u2010drying), the bioactive factors in BMCE<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>We developed a method to freeze\u2010dry BMCE<\/jats:styled-content> and then to analyze its ingredients and functions in vivo. Freeze\u2010dried (FD<\/jats:styled-content>) BMCE<\/jats:styled-content>, freshly prepared BMCE<\/jats:styled-content> (positive control), or saline (vehicle control) was injected into the tail vein of mice that had received irradiation to damage their salivary glands.<\/jats:p><\/jats:sec>Results<\/jats:title>Results demonstrated that the presence of angiogenesis\u2010related factors and cytokines in FD<\/jats:styled-content>\u2010BMCE<\/jats:styled-content> remained comparable to those found in fresh BMCE<\/jats:styled-content>. Both fresh and FD<\/jats:styled-content>\u2010BMCE<\/jats:styled-content> restored comparably saliva secretion, increased cell proliferation, upregulated regenerative\/repair genes, protected salivary acinar cells, parasympathetic nerves, and blood vessels from irradiation\u2010damaged salivary glands.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Lyophilization of BMCE<\/jats:styled-content> maintained its bioactivity and therapeutic effect on irradiation\u2010injured salivary glands. The advantages of freeze\u2010drying BMCE<\/jats:styled-content> are its storage and transport at ambient temperature.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12728","type":"journal-article","created":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T14:30:50Z","timestamp":1519655450000},"page":"202-206","source":"Crossref","is-referenced-by-count":17,"title":["Lyophilized bone marrow cell extract functionally restores irradiation\u2010injured salivary glands"],"prefix":"10.1111","volume":"24","author":[{"given":"X","family":"Su","sequence":"first","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Fang","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Liu","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Ruan","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"},{"name":"College of Chemistry and Bioengineering Guilin University of Technology Guilin Guangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Seuntjens","sequence":"additional","affiliation":[{"name":"Department of Oncology Medical Physics Unit McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JM","family":"Kinsella","sequence":"additional","affiliation":[{"name":"Department of Bioengineering McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5594-359X","authenticated-orcid":false,"given":"SD","family":"Tran","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s10895-016-2014-x","article-title":"Long shelf life of a lyophilized DNA Aptamer Beacon Assay","volume":"27","author":"Bruno J. 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