{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:10:56Z","timestamp":1781284256549,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T00:00:00Z","timestamp":1506556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Foundation of Chongqing City Health and Family Planning Committee","award":["20142050"],"award-info":[{"award-number":["20142050"]}]},{"DOI":"10.13039\/501100013223","name":"Chongqing Research Program of Basic Research and Frontier Technology","doi-asserted-by":"crossref","award":["cstc2015jcyjA10035"],"award-info":[{"award-number":["cstc2015jcyjA10035"]}],"id":[{"id":"10.13039\/501100013223","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013223","name":"Chongqing Research Program of Basic Research and Frontier Technology","doi-asserted-by":"crossref","award":["cstc2016jcyjA0200"],"award-info":[{"award-number":["cstc2016jcyjA0200"]}],"id":[{"id":"10.13039\/501100013223","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Research Program of the Chongqing Education Bureau","award":["KJ1400218"],"award-info":[{"award-number":["KJ1400218"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>In this study, we constructed a composite by combining the human dental follicle cell sheet and a manual drilled porous decalcified dentin matrix that was used to construct ectopic tissue\u2010engineered periodontal ligament\u2010like tissues in renal capsules of nude mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Human dental follicle cells were harvested from human lower third molars and then embedded into a\u00a0temperature\u2010sensitive\u00a0culture dish. These cells were then\u00a0placed into frozen porous\u00a0decalcified dentin matrix sheets and\u00a0induced by 50\u00a0g\/ml\u00a0ascorbic acid. This established a \u201csandwich\u00a0structure\u201d in vitro implant that was placed in nude mice under the renal capsule. The mice were sacrificed at 4 and 8\u00a0weeks after implantation, and\u00a0the implants were assessed after haematoxylin\u2013eosin staining,\u00a0Masson\u00a0staining and\u00a0immunohistochemical staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The experimental group showed a fibre structure\u00a0between\u00a0the dentin and <jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TCP<\/jats:styled-content> after 4\u00a0weeks. After 8\u00a0weeks, the collagen fibres\u00a0increased, and the direction was perpendicular to the\u00a0dentin. Immunohistochemistry showed positive staining in the osteopontin and periostin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The composite can induce ectopic bone and fibre formation, and the fibre had a certain directionality. Besides, the composite can maintain the stability of the periodontal ligament\u00a0width.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12734","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T07:58:15Z","timestamp":1502956695000},"page":"429-441","source":"Crossref","is-referenced-by-count":8,"title":["Periodontal ligament\u2010like tissue regeneration with drilled porous decalcified dentin\u00a0matrix sheet composite"],"prefix":"10.1111","volume":"24","author":[{"given":"G","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Orthodontics Stomatological Hospital of Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Disease and Biomedical Sciences  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing 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