{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T06:11:40Z","timestamp":1745993500749,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24791970"],"award-info":[{"award-number":["JP24791970"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>In the gingival crevice, the interaction between epithelial cells and periodontopathic bacteria is important for the development of periodontitis. <jats:italic>Treponema denticola<\/jats:italic> is a major pathogen of chronic periodontitis and possesses several virulence factors, such as major surface protein (Msp) and prolyl\u2010phenylalanine\u2010specific protease (dentilisin). Here, we investigated the behaviours of epithelial cells infected with <jats:italic>T.\u00a0denticola<\/jats:italic> by measuring the expression of interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u20101\u03b2, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, \u03b2 defensin 2 (<jats:styled-content style=\"fixed-case\">BD<\/jats:styled-content>\u20102) and heat\u2010shock protein 70 (<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Epithelial cells were infected with <jats:italic>T.\u00a0denticola<\/jats:italic> wild\u2010type strain, Msp\u2010deficient mutant or dentilisin\u2010deficient mutant, and the expression levels of the above targets were analysed by polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Infection with <jats:italic>T.\u00a0denticola<\/jats:italic> wild\u2010type strain and mutants induced the production of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70. The level of <jats:styled-content style=\"fixed-case\">BD<\/jats:styled-content>\u20102 induced by <jats:italic>T.\u00a0denticola<\/jats:italic> wild\u2010type strain at 24\u00a0hr was significantly higher than that of the dentilisin\u2010deficient mutant. The level of <jats:italic><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<\/jats:italic>\u03b2 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in the wild\u2010type strain and dentilisin\u2010deficient mutant was slightly lower than that in the uninfected control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that the levels of <jats:styled-content style=\"fixed-case\">BD<\/jats:styled-content>\u20102 were affected by Msp and dentilisin. This effect may contribute to the disruption of the response of epithelial cells to eradicate <jats:italic>T.\u00a0denticola<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12794","type":"journal-article","created":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T14:30:50Z","timestamp":1519655450000},"page":"14-18","source":"Crossref","is-referenced-by-count":5,"title":["Response of epithelial cells infected by <i>Treponema denticola<\/i>"],"prefix":"10.1111","volume":"24","author":[{"given":"E","family":"Kokubu","sequence":"first","affiliation":[{"name":"Department of Microbiology Tokyo Dental College  Tokyo Japan"},{"name":"Oral Health Science Center Tokyo Dental College  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Inoue","sequence":"additional","affiliation":[{"name":"Oral Health Science Center Tokyo Dental College  Tokyo Japan"},{"name":"Department of Clinical Pathophysiology Tokyo Dental College  Tokyo 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