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The lesions were diagnosed histopathologically as oral epithelial dysplasia, oral lichenoid dysplasia, oral lichen planus or other. Transcriptome analysis was performed by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> sequencing, hierarchical clustering, differential expression and biological pathway analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For hierarchical clustering, the samples broadly clustered according to histology rather than the margins with lichenoid samples clustering together. Differential expression analysis showed that independent of histology, there was greater molecular dysregulation between the lesion centre and autofluorescence margin compared to the lesion centre and white light margin. Furthermore, the autofluorescence and white light margins were molecularly distinct, indicating the white light margins harboured abnormality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that the molecular profile of <jats:styled-content style=\"fixed-case\">OPMD<\/jats:styled-content> changes with divergence away from the centre of the lesion, and that autofluorescence determined margins are superior to the white light margin in achieving a clear molecular margin when excising an <jats:styled-content style=\"fixed-case\">OPMD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12818","type":"journal-article","created":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T01:26:44Z","timestamp":1513301204000},"page":"732-740","source":"Crossref","is-referenced-by-count":27,"title":["Molecular classification of autofluorescence excision margins in oral potentially malignant disorders"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1642-6204","authenticated-orcid":false,"given":"C S","family":"Farah","sequence":"first","affiliation":[{"name":"Australian Centre for Oral Oncology Research &amp; Education UWA Dental School University of Western Australia  Nedlands WA Australia"},{"name":"University of Queensland Centre for Clinical Research The University of Queensland  Herston QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Kordbacheh","sequence":"additional","affiliation":[{"name":"University of Queensland Centre for Clinical Research The University of Queensland  Herston QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"John","sequence":"additional","affiliation":[{"name":"University of Queensland Centre for Clinical Research The University of Queensland  Herston QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Bennett","sequence":"additional","affiliation":[{"name":"University of Queensland Centre for Clinical Research The University of Queensland  Herston QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2042-1809","authenticated-orcid":false,"given":"S A","family":"Fox","sequence":"additional","affiliation":[{"name":"Australian Centre for Oral Oncology Research &amp; Education UWA Dental School University of Western Australia  Nedlands WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/adj.12246"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc200078p"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/194029"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.07.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_7_1","first-page":"CD004150","article-title":"Screening programmes for the early detection and prevention of oral cancer","author":"Brocklehurst P.","year":"2013","journal-title":"Cochrane Database Systematic Review"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(95)90009-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2004.07.007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2013.09.017"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2014.11.004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.23989"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.2007.00002.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21834"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10916"},{"key":"e_1_2_9_16_1","first-page":"585","article-title":"Down\u2010regulation of DMBT1 gene expression in human oral squamous cell carcinoma","volume":"15","author":"Imai M. 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