{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:49:10Z","timestamp":1773391750945,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,13]],"date-time":"2018-03-13T00:00:00Z","timestamp":1520899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE007389"],"award-info":[{"award-number":["R01DE007389"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE023342"],"award-info":[{"award-number":["R01DE023342"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,7]]},"abstract":"Objective<\/jats:title>Interleukin\u201014\u03b1\u2010transgenic (IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content>) mice develop an autoimmune exocrinopathy with characteristics similar to Sj\u00f6gren's syndrome, including sialadenitis and hyposalivation. The P2Y2<\/jats:sub> receptor (P2Y2<\/jats:sub>R) for extracellular ATP<\/jats:styled-content> and UTP<\/jats:styled-content> is upregulated during salivary gland inflammation (i.e., sialadenitis) where it regulates numerous inflammatory responses. This study investigated the role of P2Y2<\/jats:sub>Rs in autoimmune sialadenitis in the IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content> mouse model of Sj\u00f6gren's syndrome.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content> mice were bred with P2Y2<\/jats:sub>R\u2212\/\u2212<\/jats:sup> mice to generate IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content>\u00a0\u00d7\u00a0P2Y2<\/jats:sub>R\u2212\/\u2212<\/jats:sup> mice. P2Y2<\/jats:sub>R expression, lymphocytic focus scores, B\u2010 and T\u2010cell accumulation, and lymphotoxin\u2010\u03b1 expression were evaluated in the submandibular glands (SMG) along with carbachol\u2010stimulated saliva secretion in IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content>, IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content>\u00a0\u00d7\u00a0P2Y2<\/jats:sub>R\u2212\/\u2212<\/jats:sup>, and C57BL<\/jats:styled-content>\/6 control mice at 9 and 12\u00a0months of age.<\/jats:p><\/jats:sec>Results<\/jats:title>Genetic ablation of P2Y2<\/jats:sub>Rs in IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content> mice significantly reduced B and T lymphocyte infiltration of SMGs. However, reduced sialadenitis did not restore saliva secretion in IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content>\u00a0\u00d7\u00a0P2Y2<\/jats:sub>R\u2212\/\u2212<\/jats:sup> mice. Decreased sialadenitis in IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content>\u00a0\u00d7\u00a0P2Y2<\/jats:sub>R\u2212\/\u2212<\/jats:sup> mice correlated with decreased lymphotoxin\u2010\u03b1 levels, a critical proinflammatory cytokine associated with autoimmune pathology in IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content> mice.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The results of this study suggest that P2Y2<\/jats:sub>Rs contribute to the development of salivary gland inflammation in IL<\/jats:styled-content>\u201014\u03b1TG<\/jats:styled-content> mice and may also contribute to autoimmune sialadenitis in humans.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12823","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T11:45:38Z","timestamp":1514979938000},"page":"761-771","source":"Crossref","is-referenced-by-count":18,"title":["P2Y2<\/sub>R deletion ameliorates sialadenitis in IL<\/scp>\u201014\u03b1\u2010transgenic mice"],"prefix":"10.1111","volume":"24","author":[{"given":"LT","family":"Woods","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Missouri Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JM","family":"Camden","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Missouri Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MG","family":"Khalafalla","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Missouri Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MJ","family":"Petris","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Missouri Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"},{"name":"Department of Nutritional Sciences and Exercise Physiology University of Missouri Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Erb","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Missouri Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"JL","family":"Ambrus","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Allergy, Immunology and Rheumatology SUNY at Buffalo School of Medicine and Biomedical Sciences Buffalo NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3246-6353","authenticated-orcid":false,"given":"GA","family":"Weisman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Missouri Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.2.C286"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-013-8577-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38264"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm5100087"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504819200"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kew008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.4049\/jimmunol.154.1.33","article-title":"Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors","volume":"154","author":"Browning J. L.","year":"1995","journal-title":"The Journal of Immunology"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10258"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2009.10.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30381"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21936"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8171322"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803977"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00380.2013"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.14875"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2617"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1151"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600133"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.3.831"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(92)90028-A"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270830"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2010.06.011"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.122037"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1994.1123"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.2741\/2187"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2016.06.001"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.078170"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116518"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01852.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860070309"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.11.005"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.313189"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401799200"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2016.04.008"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2008.10.006"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1203440"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001520"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.8.5676"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.21591"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3220"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13953"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1103800"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.3.C1100"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00072.2012"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123641"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fodi.12823","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.12823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:40:16Z","timestamp":1695490816000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.12823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,13]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/odi.12823"],"URL":"https:\/\/doi.org\/10.1111\/odi.12823","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,13]]}}}