{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T23:00:03Z","timestamp":1776121203176,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE023348"],"award-info":[{"award-number":["R01DE023348"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Clastic cells, originating from the monocyte\u2013macrophage lineage, resorb mineralized tissues. In periapical periodontitis, alveolar bone around the tooth apex becomes resorbed; however, the roots of the teeth are often left intact by yet unknown mechanisms. Here, we examined the status of clastic cells in a periapical periodontitis model in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Periapical periodontitis was induced by performing pulp exposure on the maxillary first molar. The contralateral maxillary first molar was used as a control. The maxillae were harvested, fixed, and subjected to \u03bc<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> scanning and three\u2010dimensional volumetric analysis. <jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content> staining was performed, and osteoclasts were quantified. Immunohistochemical staining was performed for <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> OPG<\/jats:styled-content>, and F4\/80, a marker for macrophages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At the apex of the tooth, pulp exposure resulted in periapical radiolucency with mineralized tissues at the surrounding bone surfaces but not on the root surfaces. Histologically, clastic cells were present on the bone surfaces but absent around the root surfaces. Expression of F4\/80 and <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content> was not found at close proximity to the root surfaces, but <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content> was globally expressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The absence of clastic cells around the root surface of pulp\u2010exposed teeth, in part, is associated with the lack of macrophages and <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content> expression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12824","type":"journal-article","created":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T14:30:50Z","timestamp":1519655450000},"page":"57-62","source":"Crossref","is-referenced-by-count":7,"title":["Clastic cells are absent around the root surface in pulp\u2010exposed periapical periodontitis lesions in mice"],"prefix":"10.1111","volume":"24","author":[{"given":"A","family":"Shah","sequence":"first","affiliation":[{"name":"The Shapiro Family Laboratory of Viral Oncology and Aging Research UCLA School of Dentistry  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Lee","sequence":"additional","affiliation":[{"name":"The Shapiro Family Laboratory of Viral Oncology and Aging Research UCLA School of Dentistry  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry School of Dentistry Dankook University  Cheonan Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Kim","sequence":"additional","affiliation":[{"name":"The Shapiro Family Laboratory of Viral Oncology and Aging Research UCLA School of Dentistry  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MK","family":"Kang","sequence":"additional","affiliation":[{"name":"The Shapiro Family Laboratory of Viral Oncology and Aging Research UCLA School of Dentistry  Los Angeles CA USA"},{"name":"UCLA Jonsson Comprehensive Cancer Center  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5635-7728","authenticated-orcid":false,"given":"RH","family":"Kim","sequence":"additional","affiliation":[{"name":"The Shapiro Family Laboratory of Viral Oncology and Aging Research UCLA School of Dentistry  Los Angeles CA USA"},{"name":"UCLA Jonsson Comprehensive Cancer Center  Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2014.04.013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.09.007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512469026"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1985.tb00426.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-06489-7.00016-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130401500205"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092370404"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8905"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650111207"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1127"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1346.013"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00331.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00303076"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199601)244:1<33::AID-AR4>3.0.CO;2-G"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10370"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12197"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12186"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1016\/j.joen.2016.07.020","article-title":"Preexisting periapical inflammatory condition exacerbates tooth extraction\u2010induced bisphosphonate\u2010related osteonecrosis of the jaw lesions in mice","volume":"42","author":"Song M.","year":"2016","journal-title":"Journal of Endodontics"},{"key":"e_1_2_8_20_1","first-page":"65","article-title":"Osteoclast formation and differentiation: An overview","volume":"59","author":"Soysa N. 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