{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:23:56Z","timestamp":1787761436329,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,13]],"date-time":"2018-03-13T00:00:00Z","timestamp":1520899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["175075"],"award-info":[{"award-number":["175075"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,7]]},"abstract":"Objectives<\/jats:title>This study aimed to analyse Epstein\u2013Barr virus (EBV<\/jats:styled-content>) and human cytomegalovirus (HCMV<\/jats:styled-content>) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF<\/jats:styled-content>\u2010\u03b1, IL<\/jats:styled-content>\u20101\u03b2 and IL<\/jats:styled-content>\u20106 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines.<\/jats:p><\/jats:sec>Materials and Methods<\/jats:title>A total of 100 samples of apical periodontitis were subjected to HCMV<\/jats:styled-content> and EBV<\/jats:styled-content> copy numbers analysis by nested polymerase chain reaction (PCR<\/jats:styled-content>) and TaqMan real\u2010time PCR<\/jats:styled-content>. The concentrations of TNF<\/jats:styled-content>\u2010\u03b1, IL<\/jats:styled-content>\u20101\u03b2 and IL<\/jats:styled-content>\u20106 were determined by ELISA<\/jats:styled-content> method. SPSS<\/jats:styled-content> software was used for statistical analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>There were no significant differences in the occurrence of EBV<\/jats:styled-content> and HCMV<\/jats:styled-content> between symptomatic and asymptomatic periapical lesions (p<\/jats:italic>\u00a0=\u00a0.686, p<\/jats:italic>\u00a0=\u00a0.879, respectively). Only 12 of 74 EBV<\/jats:styled-content> (16.2%) and four of 54 HCMV<\/jats:styled-content> (13.5%) nested PCR<\/jats:styled-content>\u2010positive samples showed increased viral copy numbers above the limit of 125 copies\/ml. There was no significant correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The observed low viral loads point to a relatively rare occurrence of active EBV<\/jats:styled-content> and HCMV<\/jats:styled-content> infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12829","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T12:46:39Z","timestamp":1515588399000},"page":"840-846","source":"Crossref","is-referenced-by-count":21,"title":["Herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6512-4934","authenticated-orcid":false,"given":"A","family":"Jakovljevic","sequence":"first","affiliation":[{"name":"Department of Pathophysiology School of Dental Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Knezevic","sequence":"additional","affiliation":[{"name":"Department of Virology Institute of Microbiology and Immunology Faculty of Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Nikolic","sequence":"additional","affiliation":[{"name":"Department of Human Genetics School of Dental Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Soldatovic","sequence":"additional","affiliation":[{"name":"Department of Medical Statistics and Informatics Faculty of Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Jovanovic","sequence":"additional","affiliation":[{"name":"Department of Virology Institute of Microbiology and Immunology Faculty of Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Milasin","sequence":"additional","affiliation":[{"name":"Department of Human Genetics School of Dental Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Andric","sequence":"additional","affiliation":[{"name":"Clinic of Oral Surgery School of Dental Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00369.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00418.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.080644"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.12.028"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/ABEYV2VKE6DHXAAA"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.10.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.5772\/64004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-016-1955-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.08.027"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/aej.12072"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.09.017"},{"key":"e_1_2_8_13_1","first-page":"231","article-title":"Measurement of protein cytokines in tissue extracts by enzyme\u2010linked immunosorbent assays: Application to lipopolysaccharide\u2010induced differential milieu of cytokines","volume":"26","author":"Matalka K. 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