{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T22:59:58Z","timestamp":1790549998868,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T00:00:00Z","timestamp":1530662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HI12C0763"],"award-info":[{"award-number":["HI12C0763"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this study was to evaluate the effects of <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 on bone\u2010grafting procedures for the treatment of fibrous dysplasia based on in vitro and in vivo experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and Methods<\/jats:title><jats:p>Proliferation of stem cells was determined by colony\u2010forming assay, <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 assay and BrdU staining. <jats:styled-content style=\"fixed-case\">OCT<\/jats:styled-content>4 and <jats:styled-content style=\"fixed-case\">NANOG<\/jats:styled-content> expression was analysed by flow cytometry and immunocytochemistry. Osteogenic differentiation was assessed by measuring <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> activity, Alizarin red S staining and in vivo transplantation. Gene expression of the osteogenic markers, <jats:italic>osteocalcin<\/jats:italic> and <jats:italic>type 1 collagen,<\/jats:italic> was determined by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s showed few calcium deposits and low <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> activity. Bone formation by transplanted <jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s was also suppressed relative to that of normal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s. However, <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 treatment enhanced osteogenic differentiation of <jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s mixed with normal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s in vitro and in vivo. Overall, <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 treatment promoted osteogenic differentiation of <jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s mixed with normal <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In patients with <jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content>, stem cells in affected bone are influenced by the mutation, resulting in weak bone formation with the proliferation of immature osteogenic cells. Current treatment of <jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content> involves surgical removal of excess bulk lesions, which can cause facial disfigurement. Our results suggest that <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u20102 application is a good adjunctive modality to the surgical treatment of patients with <jats:styled-content style=\"fixed-case\">FD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12869","type":"journal-article","created":{"date-parts":[[2018,4,18]],"date-time":"2018-04-18T07:01:03Z","timestamp":1524034863000},"page":"1057-1067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of <scp>BMP<\/scp>\u20102 on the osteogenic differentiation of bone marrow stem cells in fibrous dysplasia"],"prefix":"10.1111","volume":"24","author":[{"given":"S","family":"Um","sequence":"first","affiliation":[{"name":"Biotooth Engineering Lab Dental Research Institute Dental Regenerative Biotechnology Department of Dental Science School of Dentistry Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HY","family":"Kim","sequence":"additional","affiliation":[{"name":"Biotooth Engineering Lab Department of Oral and Maxillofacial Surgery Craniomaxillofacial Life Science Dental Research Institute School of Dentistry Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3798-1987","authenticated-orcid":false,"given":"B\u2010M","family":"Seo","sequence":"additional","affiliation":[{"name":"Biotooth Engineering Lab Department of Oral and Maxillofacial Surgery Craniomaxillofacial Life Science Dental Research Institute School of Dentistry Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2013.05.023"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(03)00225-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3920850"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2361"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.1.120"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10143-013-0500-z"},{"key":"e_1_2_9_8_1","unstructured":"2015 University of Washington Seattle A. 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