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Growth was evaluated by colony counting, and the production of the <jats:styled-content style=\"fixed-case\">VSC<\/jats:styled-content>s hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) and methyl mercaptan (<jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>) was measured using the Oral Chroma\u2122 instrument.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mucin induced the production of H<jats:sub>2<\/jats:sub>S by both bacteria, but had a slight inhibitory effect on <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> production by <jats:italic>F.\u00a0nucleatum<\/jats:italic>. It also increased the viability of <jats:italic>F.\u00a0nucleatum<\/jats:italic>. Alpha\u2010amylase increased H<jats:sub>2<\/jats:sub>S production by <jats:italic>S.\u00a0moorei<\/jats:italic> and <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content> production by <jats:italic>F.\u00a0nucleatum<\/jats:italic>, but had no effect on H<jats:sub>2<\/jats:sub>S production by <jats:italic>F.\u00a0nucleatum<\/jats:italic>. No substance altered the viability of <jats:italic>S.\u00a0moorei<\/jats:italic>. No effects of beta\u2010defensin\u20102, estradiol, or progesterone were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The salivary stress\u2010related proteins mucin and alpha\u2010amylase altered <jats:styled-content style=\"fixed-case\">VSC<\/jats:styled-content>s production by <jats:italic>F.\u00a0nucleatum and S.\u00a0moorei,<\/jats:italic> favoring H<jats:sub>2<\/jats:sub>S production. These findings are a step toward understanding the relation between stress and increased amounts of H<jats:sub>2<\/jats:sub>S.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12890","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T09:39:22Z","timestamp":1526377162000},"page":"1358-1366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Stress\u2010related salivary proteins affect the production of volatile sulfur compounds by oral bacteria"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2911-9892","authenticated-orcid":false,"given":"Patricia Oliveira","family":"de Lima","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5964-8245","authenticated-orcid":false,"given":"Bruno Dias","family":"Nani","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Klein","family":"Marcondes","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Carlos","family":"Groppo","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Bento Alves","family":"de Moraes","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Franz\u2010Montan","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"Cogo\u2010M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Piracicaba Dental School University of Campinas \u2013 UNICAMP  Piracicaba SP Brazil"},{"name":"Faculty of Pharmaceutical Sciences University of Campinas \u2013 UNICAMP  Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31726"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.2014.552"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083564"},{"issue":"1","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1128\/iai.41.1.44-49.1983","article-title":"Effects of alpha\u2010amylase on in vitro growth of Legionella pneumophila","volume":"41","author":"Bortner C. 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