{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:54:48Z","timestamp":1778590488371,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,10]],"date-time":"2018-07-10T00:00:00Z","timestamp":1531180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["05247"],"award-info":[{"award-number":["05247"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["119585"],"award-info":[{"award-number":["119585"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002385","name":"Ministry of Higher Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002385","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2018,11]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>The human salivary gland (HSG<\/jats:styled-content>) cell line, labeled as a submandibular ductal cell line, is commonly used as in vitro models to study radiation therapy, Sj\u00f6gren's syndrome, pleomorphic adenoma, mucocele, epithelial\u2010to\u2010mesenchymal transition, and epigenetics. However, the American Type Culture Collection (ATCC<\/jats:styled-content>) has recently released a list of cross\u2010contaminated cell lines that included HSG<\/jats:styled-content>. Despite this notice, some research laboratories still use HSG<\/jats:styled-content> as a salivary cell model. Therefore, this study examined the authenticity of HSG<\/jats:styled-content> sampled from three different laboratories.<\/jats:p><\/jats:sec>Methods<\/jats:title>DNA<\/jats:styled-content> was extracted from HSG<\/jats:styled-content> and additional salivary cell lines (NS<\/jats:styled-content>\u2010SV<\/jats:styled-content>\u2010AC<\/jats:styled-content>, NS<\/jats:styled-content>\u2010SV<\/jats:styled-content>\u2010DC<\/jats:styled-content>, A253, HSY<\/jats:styled-content>) and submitted for cell line authentication with short tandem repeat (STR<\/jats:styled-content>) analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>All HSG<\/jats:styled-content> samples had STR<\/jats:styled-content> profiles indicating >80% match with HeLa in both the ATCC<\/jats:styled-content> and Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ<\/jats:styled-content>) databases. This confirmed that HSG<\/jats:styled-content> sampled from three different laboratories and HSY<\/jats:styled-content> shared a common ancestry (host) with HeLa, whereas NS<\/jats:styled-content>\u2010SV<\/jats:styled-content>\u2010AC<\/jats:styled-content>, NS<\/jats:styled-content>\u2010SV<\/jats:styled-content>\u2010DC<\/jats:styled-content>, and A253 had unique STR<\/jats:styled-content> profiles.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Short tandem repeat analysis revealed that HSG<\/jats:styled-content> was contaminated by the HeLa cell line. Furthermore, because genotyping of the original HSG<\/jats:styled-content> cell line was not performed during its establishment, it will be difficult to authenticate an uncontaminated sample of HSG<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12920","type":"journal-article","created":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T07:36:27Z","timestamp":1529480187000},"page":"1477-1483","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Cross\u2010contamination of the human salivary gland HSG<\/scp> cell line with HeLa cells: A STR<\/scp> analysis study"],"prefix":"10.1111","volume":"24","author":[{"given":"Li\u2010Chieh","family":"Lin","sequence":"first","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4875-7534","authenticated-orcid":false,"given":"Osama","family":"Elkashty","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"},{"name":"Oral Pathology Department Faculty of Dentistry Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murali","family":"Ramamoorthi","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Trinh","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Younan","family":"Liu","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9204-6513","authenticated-orcid":false,"given":"Gulshan","family":"Sunavala\u2010Dossabhoy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Health Sciences Center\u2010Shreveport Louisiana State University Shreveport Louisiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Pranzatelli","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research NIH Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew G.","family":"Michael","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research NIH Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Chivasso","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry Faculty of Medicine Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Perret","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry Faculty of Medicine Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Chiorini","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research NIH Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Delporte","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry Faculty of Medicine Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5594-359X","authenticated-orcid":false,"given":"Simon D.","family":"Tran","sequence":"additional","affiliation":[{"name":"McGill Craniofacial Tissue Engineering and Stem Cells Laboratory Faculty of Dentistry McGill University Montreal QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/10763270260424231"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2852"},{"key":"e_1_2_8_4_1","unstructured":"ATCC. (2016).STR authentication: using the ATCC public STR Database Tutorial. Retrieved fromhttps:\/\/www.atcc.org\/STR_Database.aspx?slp=1"},{"key":"e_1_2_8_5_1","unstructured":"ATCC. (2017).STR Profile Database. Retrieved fromhttps:\/\/www.atcc.org\/STR_Database.aspx?slp=1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2015-202929"},{"key":"e_1_2_8_7_1","first-page":"24","article-title":"Immortalization of normal human salivary gland cells with duct\u2010, myoepithelial\u2010, acinar\u2010, or squamous phenotype by transfection with SV40 ori\u2010 mutant deoxyribonucleic acid","volume":"69","author":"Azuma M.","year":"1993","journal-title":"Laboratory Investigation"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002256"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M910007199"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1556-4029.2006.00046.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112582"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.02.098"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25242"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041550202"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604340"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.01.022"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-5950.154812"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105231"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/51.5.1417"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi602636m"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2018.04.004"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.221648"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.22.187"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.113.005777"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.340216"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.5858\/133.9.1463","article-title":"Henrietta Lacks, HeLa cells, and cell culture contamination","volume":"133","author":"Lucey B. P.","year":"2009","journal-title":"Archives of Pathology & Laboratory Medicine"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2011.01.001"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1982.tb01291.x"},{"key":"e_1_2_8_29_1","volume-title":"STR data analysis and interpretation for forensic analysts","author":"NIJ.gov","year":"2008"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210646"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(85)80132-5"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2147\/TCRM.S70652"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0b013e3181917af0"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540104901105"},{"key":"e_1_2_8_35_1","first-page":"3878","article-title":"Intermediate\u2010sized filaments and specific markers in a human salivary gland adenocarcinoma cell line and its nude mouse tumors","volume":"45","author":"Sato M.","year":"1985","journal-title":"Cancer Research"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.11.023"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19810801)48:3<745::AID-CNCR2820480314>3.0.CO;2-7"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21387"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2007.0208"},{"key":"e_1_2_8_40_1","first-page":"329","article-title":"Cell line individualization by STR multiplex system in the cell bank found cross\u2010contamination between ECV304 and EJ\u20101\/T24","volume":"18","author":"Tanabe H.","year":"1999","journal-title":"Tissue Culture Research Communications"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.172"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2007.01.025"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02899028"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-266718"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014-0167-RA"}],"container-title":["Oral Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fodi.12920","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/odi.12920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T03:33:47Z","timestamp":1694921627000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/odi.12920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,10]]},"references-count":44,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/odi.12920"],"URL":"https:\/\/doi.org\/10.1111\/odi.12920","archive":["Portico"],"relation":{},"ISSN":["1354-523X","1601-0825"],"issn-type":[{"value":"1354-523X","type":"print"},{"value":"1601-0825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,10]]},"assertion":[{"value":"2018-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}