{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:06:04Z","timestamp":1776132364957,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T00:00:00Z","timestamp":1537833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Italy","award":["62040"],"award-info":[{"award-number":["62040"]}]},{"name":"Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Italy","award":["0039804"],"award-info":[{"award-number":["0039804"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>A preliminary study to determine collagen fibril diameter (CF\u2010ED) distribution on medial and lateral sides of cleft lip (CL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Tissue samples from medial and lateral sides of CL were fixed in 2.5% glutaraldehyde and 1% osmium tetroxide and embedded in Araldite CY212 resin for transmission electron microscopy. The analysis of CF\u2010ED was performed using the ImageJ program. To characterize the packaging of collagen fibrils (CFs) in the two tissues, we estimated the collagen number density (CF\u2010ND) and fibril\u2010area\u2010fraction (FAF). Differences in measurements across the two sides were calculated using Wilcoxon signed\u2010rank test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The CF\u2010ED was statistically significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) smaller on the medial side (45.69\u00a0\u00b1\u00a07.89\u00a0nm) than on the lateral side (54.18\u00a0\u00b1\u00a07.62\u00a0nm). The medial side had a higher CF\u2010ND and a higher percentage of FAF than the lateral side.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our finding of a smaller CF\u2010ED and higher CF\u2010ND and FAF for the medial side suggests possible differences in size and distribution of CFs between medial and lateral sides of CL. This finding provides knowledge toward underlying tissue biomechanics that may help reconstruction of perioral tissue scaffolds, ultimately resulting in better treatment of patients with oral clefts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12962","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T08:18:48Z","timestamp":1535098728000},"page":"206-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ultrastructural analysis of collagen fibril diameter distribution in cleft lip"],"prefix":"10.1111","volume":"25","author":[{"given":"Mohammad Faisal J.","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Little","sequence":"additional","affiliation":[{"name":"School of Epidemiology and Public Health University of Ottawa Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapas Chandra","family":"Nag","sequence":"additional","affiliation":[{"name":"Department of Anatomy All India Institute of Medical Sciences New Delhi India"},{"name":"Sophisticated Analytical Instrumentation Facility (SAIF), Department of Anatomy All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter Anthony","family":"Mossey","sequence":"additional","affiliation":[{"name":"Craniofacial Development at the World Health Organization\u2013Collaborating Centre for Oral and Craniofacial Research, Dental Hospital and School University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Autelitano","sequence":"additional","affiliation":[{"name":"Smile House, Regional Centre for Orofacial Clefts and Craniofacial Anomalies, Department of Cranio\u2010Maxillo\u2010Facial Surgery, San Paolo Hospital University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Costanza","family":"Meazzini","sequence":"additional","affiliation":[{"name":"Smile House, Regional Centre for Orofacial Clefts and Craniofacial Anomalies, Department of Cranio\u2010Maxillo\u2010Facial Surgery, San Paolo Hospital University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed","family":"Merajuddin","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuraag","family":"Singh","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrumentation Facility (SAIF), Department of Anatomy All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1448-9516","authenticated-orcid":false,"given":"Michele","family":"Rubini","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9290.79968"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051407"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.b.20081"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1597\/10-092"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345990780120401"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345980770080901"},{"issue":"4","key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1242\/dev.100.4.637","article-title":"Effects of chlorcyclizine\u2010induced glycosaminoglycan alterations on patterns of hyaluronate distribution during morphogenesis of the mouse secondary palate","volume":"100","author":"Brinkley L. 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