{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T11:55:45Z","timestamp":1778586945786,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T00:00:00Z","timestamp":1537833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81072375"],"award-info":[{"award-number":["81072375"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2007CB511905"],"award-info":[{"award-number":["2007CB511905"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine the possible associations between methylation changes in the promoter regions of long interspersed nucleotide element\u20101 (<jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\u20101) and interferon regulatory factor 6 gene (<jats:italic><jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>6<\/jats:italic>) and nonsyndromic cleft lip with or without cleft palate (<jats:styled-content style=\"fixed-case\">NSCL<\/jats:styled-content>\/P).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A case\u2013control investigation was performed to compare 37 infants affected by <jats:styled-content style=\"fixed-case\">NSCL<\/jats:styled-content>\/Ps with 60 babies without cleft malformations. Their genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples were obtained, and the <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\u20101 and <jats:italic><jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>6<\/jats:italic> methylation levels were measured by using Sequenom MassArray. Unconditional logistic regression was adopted to estimate the odds ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Infants with <jats:styled-content style=\"fixed-case\">NSCL<\/jats:styled-content>\/Ps had a higher methylation level at <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\u20101 and <jats:italic><jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>6<\/jats:italic> promoter regions than controls. High levels of <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\u20101 (\u226564.07%) and <jats:italic><jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>6<\/jats:italic> (\u22656.46%) methylation were associated with an increased risk of <jats:styled-content style=\"fixed-case\">NSCL<\/jats:styled-content>\/P (<jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>\u00a0=\u00a02.63, 95% CI: 1.07\u20136.57; <jats:italic><jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>6<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> OR<\/jats:styled-content>\u00a0=\u00a04.73, 95% CI: 2.10\u201313.07), and the associations remained to be significant after adjusting for potential confounders. Similar associations were also found for cleft lip only, cleft lip, and palate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggested that aberrant methylation of <jats:styled-content style=\"fixed-case\">LINE<\/jats:styled-content>\u20101 and <jats:italic><jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>6<\/jats:italic> might contribute to the development of <jats:styled-content style=\"fixed-case\">NSCL<\/jats:styled-content>\/Ps. Further studies are needed to replicate the findings.<\/jats:p><\/jats:sec>","DOI":"10.1111\/odi.12965","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T17:17:37Z","timestamp":1535476657000},"page":"215-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Association of long interspersed nucleotide element\u20101 and interferon regulatory factor 6 methylation changes with nonsyndromic cleft lip with or without cleft palate"],"prefix":"10.1111","volume":"25","author":[{"given":"Yanhua","family":"Li","sequence":"first","affiliation":[{"name":"Obstetric and Gynecologic Department West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Deng","sequence":"additional","affiliation":[{"name":"National Center for Birth Defects Monitoring West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changfei","family":"Deng","sequence":"additional","affiliation":[{"name":"National Center for Birth Defects Monitoring West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Obstetrics &amp; Gynecology and Pediatric Diseases and Birth Defects of the Ministry of Education West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Obstetrics &amp; Gynecology and Pediatric Diseases and Birth Defects of the Ministry of Education West China Second University Hospital Sichuan University Chengdu China"},{"name":"Pediatric Department West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Obstetrics &amp; Gynecology and Pediatric Diseases and Birth Defects of the Ministry of Education West China Second University Hospital Sichuan University Chengdu China"},{"name":"Pediatric Department West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Hengyang Women and Children Hospital Hengyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanmin","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Obstetrics &amp; Gynecology and Pediatric Diseases and Birth Defects of the Ministry of Education West China Second University Hospital Sichuan University Chengdu China"},{"name":"Pediatric Department West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6116-8024","authenticated-orcid":false,"given":"Li","family":"Dai","sequence":"additional","affiliation":[{"name":"National Center for Birth Defects Monitoring West China Second University Hospital Sichuan University Chengdu China"},{"name":"Key Laboratory of Obstetrics &amp; Gynecology and Pediatric Diseases and Birth Defects of the Ministry of Education West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046705"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02721-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.11-034A"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.5694\/mja12.10811"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110931108"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20670"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20607"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016252"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40572-015-0045-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.042572"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2011.10.002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph10094352"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2008.0837"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.23320"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12791"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi-2017-0081"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI72688"},{"key":"e_1_2_8_19_1","first-page":"408","article-title":"Nonsyndromic cleft lip with or without cleft palate in Chinese population: Analysis of 3766 cases","volume":"31","author":"Liang J.","year":"2000","journal-title":"Hua Xi Yi Ke Da Xue Xue Bao"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033290"},{"key":"e_1_2_8_21_1","volume-title":"National stocktaking report on birth defect prevention (2012)","author":"MOH","year":"2012"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0004.2002.610402.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.53.3-4.240"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2014.998536"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-017-0362-2"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-12-31"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-8794-5-20"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022015000200037"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078462"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-150490"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.20117"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.28858"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.23540"},{"key":"e_1_2_8_34_1","first-page":"534","article-title":"Pre\u2010conception folic acid and multivitamin supplementation for the primary and secondary prevention of neural tube defects and other folic acid\u2010sensitive congenital anomalies","volume":"37","author":"Wilson R. 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