{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:13Z","timestamp":1787015953034,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T00:00:00Z","timestamp":1542240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K15760"],"award-info":[{"award-number":["15K15760"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Orthognathic surgery dramatically changes morphology of the maxillofacial deformity and improves the malocclusion morphologically and functionally. We investigated the influence of orthognathic surgery on genomewide DNA methylation in saliva.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Saliva was obtained from nine patients undergoing orthognathic surgery and two healthy reference individuals before and 3\u00a0months after orthognathic surgery. Genomewide DNA methylation profiling of saliva (341,482 CpG dinucleotides) was conducted using Infinium HumanMethylation450 BeadChips.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparison between pre\u2010 and postsurgery saliva samples revealed significant changes in DNA methylation patterns at 2,381 CpG sites (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01) with suggestive significance. The differentially methylated probe sets were significantly associated with the cancer pathway (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a02.8\u00a0\u00d7\u00a010\n                      <jats:sup>\u22127<\/jats:sup>\n                      ; a false discovery rate\n                      <jats:italic>q<\/jats:italic>\n                      \u2010value\u00a0=\u00a03.7\u00a0\u00d7\u00a010\n                      <jats:sup>\u22124<\/jats:sup>\n                      ) and PI3K\u2010Akt signalling pathway (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a02.4\u00a0\u00d7\u00a010\n                      <jats:sup>\u22125<\/jats:sup>\n                      ; a false discovery rate\n                      <jats:italic>q<\/jats:italic>\n                      \u2010value\u00a0=\u00a03.1\u00a0\u00d7\u00a010\n                      <jats:sup>\u22122<\/jats:sup>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Pathway enrichment analysis of genes with suggestive significance demonstrated that altered DNA methylation in saliva of patients undergoing orthognathic surgery, possibly as a response to surgical stress or bone injury. Further studies with a large sample size and long\u2010term observation are needed to validate the phenomena identified in this study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.12998","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T07:54:44Z","timestamp":1540540484000},"page":"508-514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Orthognathic surgery induces genomewide changes longitudinally in DNA methylation in saliva"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9806-7163","authenticated-orcid":false,"given":"Tetsutaro","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Department of Orthodontics Showa University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Hosomichi","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics 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