{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:26:41Z","timestamp":1790429201202,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T00:00:00Z","timestamp":1544918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["PAPIIT IA208016"],"award-info":[{"award-number":["PAPIIT IA208016"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oral Diseases"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the expression of proteins related to activation of the NLRP3 inflammasome in patients with chronic periodontitis (CP) and type 2 diabetes mellitus (T2D), and to determine whether the exacerbated periodontal pathological process observed in diabetic patients is related to its upregulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We performed an observational, analytical, cross\u2010sectional study in three study groups: individuals systemically and orally healthy, and patients with CP with and without T2D. Gingival biopsies were taken from the three study groups. The expression of mRNAs for\n                      <jats:italic>CASP1<\/jats:italic>\n                      ,\n                      <jats:italic>NLRP3<\/jats:italic>\n                      and\n                      <jats:italic>ASC<\/jats:italic>\n                      was detected using real\u2010time PCR, and the expression of NLRP3 and ASC proteins was determined by immunohistochemistry. The quantification of IL\u201018 and IL\u20101\u03b2 was determined in the gingival crevicular fluid using ELISA. The results were analysed by ANOVA followed by Tukey's test to compare differences between individual groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Patients with CP and uncontrolled T2D presented severe periodontal disease and inflammation (PPD,\n                      <jats:italic>p\u00a0=\u00a0<\/jats:italic>\n                      0.0072; CAL,\n                      <jats:italic>p\u00a0=\u00a0<\/jats:italic>\n                      0.0480; bone loss,\n                      <jats:italic>p\u00a0=\u00a0<\/jats:italic>\n                      0.0088), higher levels of\n                      <jats:italic>CASP1<\/jats:italic>\n                      mRNA expression (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.0026), a stronger pattern of staining for NLRP3 and ASC proteins in the epithelium and connective tissues, and significantly higher production of IL\u201018 (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.0063) and IL\u20101\u03b2 (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.0018) in comparison with healthy or CP subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The upregulation of genes and proteins involved in the activation of the NLRP3 inflammasome components in patients with periodontitis and uncontrolled T2D suggests a possible role in the more severe pathological processes leading to destruction of periodontal tissues observed in these patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/odi.13003","type":"journal-article","created":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T10:22:48Z","timestamp":1542104568000},"page":"596-608","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Upregulation of proteins of the NLRP3 inflammasome in patients with periodontitis and uncontrolled type 2 diabetes"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7682-6878","authenticated-orcid":false,"given":"Ana Lilia","family":"Garc\u00eda\u2010Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Odontol\u00f3gica, Secci\u00f3n Inmunidad Oral y Regulaci\u00f3n \u00d3sea, FES Iztacala  UNAM Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel Enrique","family":"Mu\u00f1oz\u2010Saavedra","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Odontol\u00f3gica, Secci\u00f3n Inmunidad Oral y Regulaci\u00f3n \u00d3sea, FES Iztacala  UNAM Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Gonz\u00e1lez\u2010Alva","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioingenier\u00eda de Tejidos, Facultad de Odontolog\u00eda UNAM, Ciudad Universitaria Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Moreno\u2010Fierros","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunidad en Mucosas UBIMED, FES Iztacala UNAM Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulgencio Eduardo","family":"Llamosas\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Odontol\u00f3gica, Secci\u00f3n Inmunidad Oral y Regulaci\u00f3n \u00d3sea, FES Iztacala  UNAM Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul Ernesto","family":"Cifuentes\u2010Mendiola","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Odontol\u00f3gica, Secci\u00f3n Inmunidad Oral y Regulaci\u00f3n \u00d3sea, FES Iztacala  UNAM Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nestor","family":"Rubio\u2010Infante","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunidad en Mucosas UBIMED, FES Iztacala UNAM Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,16]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"77","article-title":"Type 2 diabetes mellitus and periodontal disease severity","volume":"12","author":"Al\u2010Khabbaz A. 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